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AB132787

Recombinant Human PNPLA3 protein

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Recombinant Human PNPLA3 protein is a Human Full Length protein, in the 1 to 481 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ADPN, C22orf20, PNPLA3, 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3, Acylglycerol transacylase, Adiponutrin, Calcium-independent phospholipase A2-epsilon, Lysophosphatidic acid acyltransferase, Patatin-like phospholipase domain-containing protein 3, iPLA2-epsilon

1 Images
SDS-PAGE - Recombinant Human PNPLA3 protein (AB132787)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PNPLA3 protein (AB132787)

12.5% SDS-PAGE analysis of ab132787 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q9NST1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLSDLVRKARSRNIGIFHPSFNLSKFLRQGLGKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVDALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKLSLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRIMSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPEGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL","proteinLength":"Full Length","predictedMolecularWeight":"79.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":481,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NST1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNPLA3 also known as patatin-like phospholipase domain-containing protein 3 is an enzyme that catalyzes the hydrolysis of ester bonds in glycerophospholipids. This protein has a molecular mass of about 53 kDa and is mainly expressed in liver tissue as well as in adipose tissue. It plays a role in lipid remodeling and triglyceride metabolism. The gene for PNPLA3 is located on chromosome 22 and its expression levels can influence its function in cellular lipid dynamics.
Biological function summary

PNPLA3 impacts lipid metabolism and storage in the liver. It does not function as part of a complex but rather exerts its effects independently. PNPLA3 modifies lipid droplet contents by converting triglyceride substrates. This activity helps regulate lipolysis affecting lipid droplet size and number in liver cells. Its function ensures a balance in hepatic lipid levels which is important for maintaining proper liver function and overall metabolic health.

Pathways

PNPLA3 is integral to lipid metabolic pathways and energy homeostasis. It interacts with key players in triglyceride metabolism such as DGAT2 and PNPLA2. These proteins especially DGAT2 help in the transformation and storage of triglycerides reflecting PNPLA3's role in regulating lipid balance. PNPLA3's interactions and functions in these pathways highlight its part in energy storage and utilization processes.

PNPLA3 genetic variants associate with non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Its role in these conditions relates to its impact on liver triglyceride content. The I148M variant of PNPLA3 has a notable connection to an increased risk of developing NAFLD. Additionally PNPLA3 interacts with proteins involved in fibrogenesis pathways such as TGF-β which influences the progression of liver disease towards fibrosis. Understanding these interactions helps researchers develop targeted therapies for liver conditions.
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Specifications

Form

Liquid

Additional notes

Glutathione Sepharose 4 Fast Flow

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General info

Function

Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs : arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed : 22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed : 15364929, PubMed : 20034933, PubMed : 22560221). In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety (PubMed : 21878620). However, the triacylglycerol hydrolase activity is controversial and may be very low (PubMed : 22560221). Possesses phospholipase A2 activity (PubMed : 15364929).

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Product protocols

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Target data

Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs : arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed : 22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed : 15364929, PubMed : 20034933, PubMed : 22560221). In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety (PubMed : 21878620). However, the triacylglycerol hydrolase activity is controversial and may be very low (PubMed : 22560221). Possesses phospholipase A2 activity (PubMed : 15364929).
See full target information PNPLA3
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