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AB101213

Recombinant Human PNPO protein

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Recombinant Human PNPO protein is a Human Fragment protein, in the 57 to 261 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Pyridoxine-5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, PNPO

1 Images
SDS-PAGE - Recombinant Human PNPO protein (AB101213)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PNPO protein (AB101213)

SDS-PAGE of ab101213 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NVS9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP","proteinLength":"Fragment","predictedMolecularWeight":"25.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":57,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NVS9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PNPO protein also known as pyridoxine 5'-phosphate oxidase plays a mechanical role in the biosynthesis of pyridoxal 5'-phosphate (PLP) the active form of vitamin B6. This enzyme with a mass of approximately 30 kDa catalyzes the oxidation of pyridoxine 5'-phosphate (PNP) to PLP. PNPO is expressed widely in human tissues particularly in the liver kidney and brain where vitamin B6 metabolism is essential.
Biological function summary

PNPO contributes to the production of PLP a cofactor involved in numerous enzymatic reactions including neurotransmitter synthesis and amino acid metabolism. PLP acts as a critical helper molecule in the functioning of over 140 enzymes. PNPO operates as part of a multi-step enzyme complex responsible for the conversion of dietary vitamin B6 forms into PLP ensuring the availability of this vital coenzyme throughout the body.

Pathways

PNPO integrates into the vitamin B6 metabolism pathway which is important for maintaining cellular functions. This enzyme assures the proper transformation of PNP to PLP coordinating with other enzymes such as pyridoxal kinase to regulate levels of active vitamin B6. PNPO's activity influences pathways like the transsulfuration and tryptophan degradation which rely on PLP-dependent enzymes.

PNPO deficiencies can lead to neurological conditions such as epilepsy due to impaired PLP production which affects neurotransmitter synthesis. Mutations in the PNPO gene may result in an inborn error of metabolism. In some cases PNPO interacts with other proteins such as glutamic acid decarboxylase which depend on PLP for activity. These interactions can exacerbate disease phenotypes highlighting PNPO's importance in maintaining neurological health.
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Specifications

Form

Liquid

Additional notes

ab101213 was purified using conventional chromatography.

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General info

Function

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).

Sequence similarities

Belongs to the pyridoxamine 5'-phosphate oxidase family.

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Product protocols

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Target data

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
See full target information PNPO
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