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Recombinant Human PNUTS protein is a Human Fragment protein, in the 661 to 929 aa range, expressed in Escherichia coli, with =90% purity and suitable for SDS-PAGE.

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Key facts

Purity

=90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers (By similarity).

Alternative names

Recommended products

Recombinant Human PNUTS protein is a Human Fragment protein, in the 661 to 929 aa range, expressed in Escherichia coli, with =90% purity and suitable for SDS-PAGE.

Key facts

Purity

=90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q96QC0-1
Animal free

No

Species

Human

Reconstitution

Reconstitute in water

Concentration
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Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

Sequence info

Amino acid sequence

Accession

Q96QC0

Protein length

Fragment

Predicted molecular weight

26.3 kDa

Amino acids

661 to 929

Nature

Recombinant

Tags

His-DHFR tag N-Terminus

Specifications

Form

Lyophilized

Additional notes

Purified via His tag

General info

Function

Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers (By similarity).

Post-translational modifications

Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes

This product was previously labelled as PPP1R10

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The PNUTS protein also known as 'little PNUTS' acts as a regulatory subunit in the cell. It plays a role in controlling the activity of protein phosphatase 1 (PP1) essential for various cellular processes. The PNUTS protein has a molecular mass of approximately 106 kDa. It is expressed in multiple tissues with significant presence in the brain and heart suggesting its importance in these areas.

Biological function summary

The PNUTS protein influences many cellular events as it is part of a larger complex. It controls the dephosphorylation activity of PP1 impacting processes like cell cycle regulation and chromatin structure modification. This protein contributes to the dynamic regulation of transcription DNA repair and cell survival. Its ability to interact with other proteins positions it as a versatile component in cell biology.

Pathways

The PNUTS protein interacts in several notable pathways that affect cell function. It is involved in the DNA damage response pathway where it regulates DNA repair through its interaction with components like PP1. It also plays a role in the Rb signaling pathway related to cell cycle progression linking its function to cell growth and division. Through these pathways PNUTS connects with other proteins emphasizing its versatility.

Associated diseases and disorders

Alterations in the PNUTS protein correlate with various conditions. Its dysfunction is associated with cancer impacting cell proliferation through its role in the Rb pathway. The connection between PNUTS and neurodegenerative disorders also emerges due to its high expression in the brain. In diseases like Alzheimer's PNUTS interactions with proteins like tau influence disease progression making it a target of interest in therapeutic research.

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