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AB127514

Recombinant Human PNUTS protein (His)

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Recombinant Human PNUTS protein (His) is a Human Fragment protein, in the 661 to 929 aa range, expressed in Escherichia coli, with 90%, suitable for SDS-PAGE.

View Alternative Names

CAT53, FB19, PNUTS, PPP1R10, Serine/threonine-protein phosphatase 1 regulatory subunit 10, MHC class I region proline-rich protein CAT53, PP1-binding protein of 114 kDa, Phosphatase 1 nuclear targeting subunit, p99

Key facts

Purity

90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96QC0

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Complementary Products

Primary Antibodies

AB249748

Anti-PNUTS antibody [EPR11706] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] - BSA and Azide free (AB249748)

  • 0
  • 0
Primary Antibodies

AB133747

Anti-ULK1 (phospho S556) antibody [EPR6156]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)

  • 0
  • 0
Primary Antibodies

AB28179

Anti-TFIIF antibody

ChIP - Anti-TFIIF antibody (AB28179)

  • 5
  • 2
Proteins & Peptides

AB123172

Recombinant Human GGH protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GGH protein (His tag N-Terminus) (AB123172)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PPP1R10
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"26.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":929,"aminoAcidStart":661,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q96QC0","tags":[{"tag":"His","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PNUTS protein also known as 'little PNUTS' acts as a regulatory subunit in the cell. It plays a role in controlling the activity of protein phosphatase 1 (PP1) essential for various cellular processes. The PNUTS protein has a molecular mass of approximately 106 kDa. It is expressed in multiple tissues with significant presence in the brain and heart suggesting its importance in these areas.
Biological function summary

The PNUTS protein influences many cellular events as it is part of a larger complex. It controls the dephosphorylation activity of PP1 impacting processes like cell cycle regulation and chromatin structure modification. This protein contributes to the dynamic regulation of transcription DNA repair and cell survival. Its ability to interact with other proteins positions it as a versatile component in cell biology.

Pathways

The PNUTS protein interacts in several notable pathways that affect cell function. It is involved in the DNA damage response pathway where it regulates DNA repair through its interaction with components like PP1. It also plays a role in the Rb signaling pathway related to cell cycle progression linking its function to cell growth and division. Through these pathways PNUTS connects with other proteins emphasizing its versatility.

Alterations in the PNUTS protein correlate with various conditions. Its dysfunction is associated with cancer impacting cell proliferation through its role in the Rb pathway. The connection between PNUTS and neurodegenerative disorders also emerges due to its high expression in the brain. In diseases like Alzheimer's PNUTS interactions with proteins like tau influence disease progression making it a target of interest in therapeutic research.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Substrate-recognition component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation (PubMed : 39603239, PubMed : 39603240). Promoter-proximal pausing by RNA polymerase II is a transcription halt following transcription initiation but prior to elongation, which acts as a checkpoint to control that transcripts are favorably configured for transcriptional elongation (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex mediates the release of RNA polymerase II from promoter-proximal region of genes by catalyzing dephosphorylation of proteins involved in transcription, such as AFF4, CDK9, MEPCE, INTS12, NCBP1, POLR2M/GDOWN1 and SUPT6H (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex also regulates RNA polymerase II transcription termination by mediating dephosphorylation of SUPT5H in termination zones downstream of poly(A) sites, thereby promoting deceleration of RNA polymerase II transcription (PubMed : 31677974). PNUTS-PP1 complex is also involved in the response to replication stress by mediating dephosphorylation of POLR2A at 'Ser-5' of the CTD, promoting RNA polymerase II degradation (PubMed : 33264625). The PNUTS-PP1 complex also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (By similarity). PNUTS-PP1 complex mediates dephosphorylation of MYC, promoting MYC stability by preventing MYC ubiquitination by the SCF(FBXW7) complex (PubMed : 30158517). In addition to acts as a substrate-recognition component, PPP1R10/PNUTS also acts as a nuclear targeting subunit for the PNUTS-PP1 complex (PubMed : 9450550). In some context, PPP1R10/PNUTS also acts as an inhibitor of protein phosphatase 1 (PP1) activity by preventing access to substrates, such as RB (PubMed : 18360108).

Post-translational modifications

Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Substrate-recognition component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation (PubMed : 39603239, PubMed : 39603240). Promoter-proximal pausing by RNA polymerase II is a transcription halt following transcription initiation but prior to elongation, which acts as a checkpoint to control that transcripts are favorably configured for transcriptional elongation (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex mediates the release of RNA polymerase II from promoter-proximal region of genes by catalyzing dephosphorylation of proteins involved in transcription, such as AFF4, CDK9, MEPCE, INTS12, NCBP1, POLR2M/GDOWN1 and SUPT6H (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex also regulates RNA polymerase II transcription termination by mediating dephosphorylation of SUPT5H in termination zones downstream of poly(A) sites, thereby promoting deceleration of RNA polymerase II transcription (PubMed : 31677974). PNUTS-PP1 complex is also involved in the response to replication stress by mediating dephosphorylation of POLR2A at 'Ser-5' of the CTD, promoting RNA polymerase II degradation (PubMed : 33264625). The PNUTS-PP1 complex also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (By similarity). PNUTS-PP1 complex mediates dephosphorylation of MYC, promoting MYC stability by preventing MYC ubiquitination by the SCF(FBXW7) complex (PubMed : 30158517). In addition to acts as a substrate-recognition component, PPP1R10/PNUTS also acts as a nuclear targeting subunit for the PNUTS-PP1 complex (PubMed : 9450550). In some context, PPP1R10/PNUTS also acts as an inhibitor of protein phosphatase 1 (PP1) activity by preventing access to substrates, such as RB (PubMed : 18360108).
See full target information PPP1R10
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