Recombinant Human POGLUT1 protein (His tag) is a Human Full Length protein, in the 24 to 392 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
>90% SDS-PAGE
< 1 EU/µg
Baculovirus infected insect cells
His tag C-Terminus
SDS-PAGE
No
R Q K E S G S K W K V F I D Q I N R S L E N Y E P C S S Q N C S C Y H G V I E E D L T P F R G G I S R K M M A E V V R R K L G T H Y Q I T K N R L Y R E N D C M F P S R C S G V E H F I L E V I G R L P D M E M V I N V R D Y P Q V P K W M E P A I P V F S F S K T S E Y H D I M Y P A W T F W E G G P A V W P I Y P T G L G R W D L F R E D L V R S A A Q W P W K K K N S T A Y F R G S R T S P E R D P L I L L S R K N P K L V D A E Y T K N Q A W K S M K D T L G K P A A K D V H L V D H C K Y K Y L F N F R G V A A S F R F K H L F L C G S L V F H V G D E W L E F F Y P Q L K P W V H Y I P V K T D L S N V Q E L L Q F V K A N D D V A Q E I A E R G S Q F I R N H L Q M D D I T C Y W E N L L S E Y S K F L S Y N V T R R K G Y D Q I I P K M L K T E L L E H H H H H H
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes MW: 40-57 kDa (SDS–PAGE under reducing conditions) |
Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed:27807076). Notch glucosylation does not affect Notch ligand binding (PubMed:21490058). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).
C3orf9, CLP46, KTELC1, MDSRP, MDS010, UNQ490/PRO1006, C3orf9, POGLUT1, CLP46, UNQ490/PRO1006, KTELC1, MDSRP, MDS010, Protein O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase Rumi homolog, Protein O-xylosyltransferase POGLUT1, hCLP46, hRumi
Recombinant Human POGLUT1 protein (His tag) is a Human Full Length protein, in the 24 to 392 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
>90% SDS-PAGE
< 1 EU/µg
Baculovirus infected insect cells
His tag C-Terminus
SDS-PAGE
No
No
Human
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
R Q K E S G S K W K V F I D Q I N R S L E N Y E P C S S Q N C S C Y H G V I E E D L T P F R G G I S R K M M A E V V R R K L G T H Y Q I T K N R L Y R E N D C M F P S R C S G V E H F I L E V I G R L P D M E M V I N V R D Y P Q V P K W M E P A I P V F S F S K T S E Y H D I M Y P A W T F W E G G P A V W P I Y P T G L G R W D L F R E D L V R S A A Q W P W K K K N S T A Y F R G S R T S P E R D P L I L L S R K N P K L V D A E Y T K N Q A W K S M K D T L G K P A A K D V H L V D H C K Y K Y L F N F R G V A A S F R F K H L F L C G S L V F H V G D E W L E F F Y P Q L K P W V H Y I P V K T D L S N V Q E L L Q F V K A N D D V A Q E I A E R G S Q F I R N H L Q M D D I T C Y W E N L L S E Y S K F L S Y N V T R R K G Y D Q I I P K M L K T E L L E H H H H H H
Full Length
44.5 kDa
24 to 392
Recombinant
His tag C-Terminus
Liquid
Affinity purified
Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed:27807076). Notch glucosylation does not affect Notch ligand binding (PubMed:21490058). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).
Belongs to the glycosyltransferase 90 family.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Previously labelled as KTEL1.
This supplementary information is collated from multiple sources and compiled automatically.
Protein O-glucosyltransferase 1 (POGLUT1) also known as Rumi or KTELC1 catalyzes the addition of O-linked glucose to serine residues within epidermal growth factor-like (EGF) repeats. This enzyme plays an important role in protein glycosylation. POGLUT1 possesses an approximate molecular mass of 47 kDa. It predominantly exists in the endoplasmic reticulum of cells. Expression of POGLUT1 occurs in various tissues with notable levels in the heart placenta and skeletal muscle.
This enzyme modifies certain proteins through glycosylation a process vital for normal protein folding and function. POGLUT1 acts independently rather than as part of a larger protein complex. The glycosylation by POGLUT1 affects cell-cell signaling which is essential in developing tissues. It modifies proteins that control cellular signaling pathways providing structural stability and facilitating correct protein interactions.
POGLUT1 functions within the Notch signaling pathway. This pathway controls numerous cell differentiation processes impacting development and cell fate decisions. POGLUT1's glycosylation activity enhances Notch receptor maturation influencing its binding affinity and signaling. Perturbations in this pathway can also involve related proteins such as Fringe proteins which adjust Notch signaling through additional glycosylation mechanisms.
Mutations or dysregulation of POGLUT1 link to Dowling-Degos disease a skin condition characterized by reticulate pigmentation. Alterations in POGLUT1 activity may disrupt normal Notch signaling leading to abnormal skin cell differentiation and proliferation. Additionally changes in POGLUT1 function connect to congenital heart defects associating with misregulated Notch signaling and downstream effects on cardiac development.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
15% SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (ab219279) at 3 μg.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com