Skip to main content

Recombinant Human POGLUT1 protein (His tag) is a Human Full Length protein, in the 24 to 392 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (AB219279), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

R Q K E S G S K W K V F I D Q I N R S L E N Y E P C S S Q N C S C Y H G V I E E D L T P F R G G I S R K M M A E V V R R K L G T H Y Q I T K N R L Y R E N D C M F P S R C S G V E H F I L E V I G R L P D M E M V I N V R D Y P Q V P K W M E P A I P V F S F S K T S E Y H D I M Y P A W T F W E G G P A V W P I Y P T G L G R W D L F R E D L V R S A A Q W P W K K K N S T A Y F R G S R T S P E R D P L I L L S R K N P K L V D A E Y T K N Q A W K S M K D T L G K P A A K D V H L V D H C K Y K Y L F N F R G V A A S F R F K H L F L C G S L V F H V G D E W L E F F Y P Q L K P W V H Y I P V K T D L S N V Q E L L Q F V K A N D D V A Q E I A E R G S Q F I R N H L Q M D D I T C Y W E N L L S E Y S K F L S Y N V T R R K G Y D Q I I P K M L K T E L L E H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

MW: 40-57 kDa (SDS–PAGE under reducing conditions)

Target data

Function

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed:27807076). Notch glucosylation does not affect Notch ligand binding (PubMed:21490058). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).

Alternative names

Recommended products

Recombinant Human POGLUT1 protein (His tag) is a Human Full Length protein, in the 24 to 392 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Applications

SDS-PAGE

Accession
Q8NBL1-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

R Q K E S G S K W K V F I D Q I N R S L E N Y E P C S S Q N C S C Y H G V I E E D L T P F R G G I S R K M M A E V V R R K L G T H Y Q I T K N R L Y R E N D C M F P S R C S G V E H F I L E V I G R L P D M E M V I N V R D Y P Q V P K W M E P A I P V F S F S K T S E Y H D I M Y P A W T F W E G G P A V W P I Y P T G L G R W D L F R E D L V R S A A Q W P W K K K N S T A Y F R G S R T S P E R D P L I L L S R K N P K L V D A E Y T K N Q A W K S M K D T L G K P A A K D V H L V D H C K Y K Y L F N F R G V A A S F R F K H L F L C G S L V F H V G D E W L E F F Y P Q L K P W V H Y I P V K T D L S N V Q E L L Q F V K A N D D V A Q E I A E R G S Q F I R N H L Q M D D I T C Y W E N L L S E Y S K F L S Y N V T R R K G Y D Q I I P K M L K T E L L E H H H H H H

Accession

Q8NBL1

Protein length

Full Length

Predicted molecular weight

44.5 kDa

Amino acids

24 to 392

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified

General info

Function

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076, PubMed:28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed:27807076). Notch glucosylation does not affect Notch ligand binding (PubMed:21490058). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).

Sequence similarities

Belongs to the glycosyltransferase 90 family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Previously labelled as KTEL1.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein O-glucosyltransferase 1 (POGLUT1) also known as Rumi or KTELC1 catalyzes the addition of O-linked glucose to serine residues within epidermal growth factor-like (EGF) repeats. This enzyme plays an important role in protein glycosylation. POGLUT1 possesses an approximate molecular mass of 47 kDa. It predominantly exists in the endoplasmic reticulum of cells. Expression of POGLUT1 occurs in various tissues with notable levels in the heart placenta and skeletal muscle.

Biological function summary

This enzyme modifies certain proteins through glycosylation a process vital for normal protein folding and function. POGLUT1 acts independently rather than as part of a larger protein complex. The glycosylation by POGLUT1 affects cell-cell signaling which is essential in developing tissues. It modifies proteins that control cellular signaling pathways providing structural stability and facilitating correct protein interactions.

Pathways

POGLUT1 functions within the Notch signaling pathway. This pathway controls numerous cell differentiation processes impacting development and cell fate decisions. POGLUT1's glycosylation activity enhances Notch receptor maturation influencing its binding affinity and signaling. Perturbations in this pathway can also involve related proteins such as Fringe proteins which adjust Notch signaling through additional glycosylation mechanisms.

Associated diseases and disorders

Mutations or dysregulation of POGLUT1 link to Dowling-Degos disease a skin condition characterized by reticulate pigmentation. Alterations in POGLUT1 activity may disrupt normal Notch signaling leading to abnormal skin cell differentiation and proliferation. Additionally changes in POGLUT1 function connect to congenital heart defects associating with misregulated Notch signaling and downstream effects on cardiac development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (ab219279), expandable thumbnail

    SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (ab219279)

    15% SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (ab219279) at 3 μg.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com