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AB219279

Recombinant Human POGLUT1 protein (His tag)

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Recombinant Human POGLUT1 protein (His tag) is a Human Full Length protein, in the 24 to 392 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

C3orf9, CLP46, KTELC1, MDSRP, MDS010, UNQ490/PRO1006, POGLUT1, Protein O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase Rumi homolog, Protein O-xylosyltransferase POGLUT1, hCLP46, hRumi

1 Images
SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (AB219279)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (AB219279)

15% SDS-PAGE - Recombinant Human POGLUT1 protein (His tag) (ab219279) at 3 μg.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8NBL1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>MW: 40-57 kDa (SDS–PAGE under reducing conditions)</p>" } } }
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Product details

Previously labelled as KTEL1.
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Sequence info

[{"sequence":"RQKESGSKWKVFIDQINRSLENYEPCSSQNCSCYHGVIEEDLTPFRGGISRKMMAEVVRRKLGTHYQITKNRLYRENDCMFPSRCSGVEHFILEVIGRLPDMEMVINVRDYPQVPKWMEPAIPVFSFSKTSEYHDIMYPAWTFWEGGPAVWPIYPTGLGRWDLFREDLVRSAAQWPWKKKNSTAYFRGSRTSPERDPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLVDHCKYKYLFNFRGVAASFRFKHLFLCGSLVFHVGDEWLEFFYPQLKPWVHYIPVKTDLSNVQELLQFVKANDDVAQEIAERGSQFIRNHLQMDDITCYWENLLSEYSKFLSYNVTRRKGYDQIIPKMLKTELLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"44.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":392,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"Q8NBL1","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein O-glucosyltransferase 1 (POGLUT1) also known as Rumi or KTELC1 catalyzes the addition of O-linked glucose to serine residues within epidermal growth factor-like (EGF) repeats. This enzyme plays an important role in protein glycosylation. POGLUT1 possesses an approximate molecular mass of 47 kDa. It predominantly exists in the endoplasmic reticulum of cells. Expression of POGLUT1 occurs in various tissues with notable levels in the heart placenta and skeletal muscle.
Biological function summary

This enzyme modifies certain proteins through glycosylation a process vital for normal protein folding and function. POGLUT1 acts independently rather than as part of a larger protein complex. The glycosylation by POGLUT1 affects cell-cell signaling which is essential in developing tissues. It modifies proteins that control cellular signaling pathways providing structural stability and facilitating correct protein interactions.

Pathways

POGLUT1 functions within the Notch signaling pathway. This pathway controls numerous cell differentiation processes impacting development and cell fate decisions. POGLUT1's glycosylation activity enhances Notch receptor maturation influencing its binding affinity and signaling. Perturbations in this pathway can also involve related proteins such as Fringe proteins which adjust Notch signaling through additional glycosylation mechanisms.

Mutations or dysregulation of POGLUT1 link to Dowling-Degos disease a skin condition characterized by reticulate pigmentation. Alterations in POGLUT1 activity may disrupt normal Notch signaling leading to abnormal skin cell differentiation and proliferation. Additionally changes in POGLUT1 function connect to congenital heart defects associating with misregulated Notch signaling and downstream effects on cardiac development.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed : 27807076). Notch glucosylation does not affect Notch ligand binding (PubMed : 21490058). Required during early development to promote gastrulation : acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).

Sequence similarities

Belongs to the glycosyltransferase 90 family.

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Product protocols

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Target data

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed : 27807076). Notch glucosylation does not affect Notch ligand binding (PubMed : 21490058). Required during early development to promote gastrulation : acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).
See full target information POGLUT1
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