Recombinant Human POLD4 protein is a Human Full Length protein, in the 1 to 107 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G R K R L I T D S Y P V V K R R E G P A G H S K G E L A P E L G E E P Q P R D E E E A E L E L L R Q F D L A W Q Y G P C T G I T R L Q R W C R A K Q M G L E P P P E V W Q V L K T H P G D P R F Q C S L W H L Y P L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).
Alternative names
POLDS, POLD4, DNA polymerase delta subunit 4, DNA polymerase delta subunit p12
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Recombinant Human POLD4 protein is a Human Full Length protein, in the 1 to 107 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9HCU8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G R K R L I T D S Y P V V K R R E G P A G H S K G E L A P E L G E E P Q P R D E E E A E L E L L R Q F D L A W Q Y G P C T G I T R L Q R W C R A K Q M G L E P P P E V W Q V L K T H P G D P R F Q C S L W H L Y P L
- Accession
- Q9HCU8
- Protein length
- Full Length
- Predicted molecular weight
- 14.8 kDa
- Amino acids
- 1 to 107
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab171583 was purified using conventional chromatography.
General info
- Function
As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).
- Sequence similarities
Belongs to the DNA polymerase delta subunit 4 family.
- Post-translational modifications
Ubiquitinated; undergoes 'Lys-48'-linked ubiquitination in response to UV irradiation, leading to proteasomal degradation (PubMed:16934752, PubMed:17317665, PubMed:23233665, PubMed:23913683). This modification is partly mediated by RNF8 and by the DCX(DTL) E3 ubiquitin ligase complex (also called CRL4(CDT2)) (PubMed:23233665, PubMed:24022480). Efficient degradation requires the presence of PCNA and is required for the inhibition of fork progression after DNA damage (PubMed:24022480).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLD4 also known as DNA polymerase delta subunit 4 functions as a component of the DNA polymerase delta complex assisting in DNA replication and repair mechanisms. This protein has a molecular mass of approximately 12 kDa and is expressed in various tissues including those with high proliferative rates such as the spleen and thymus. As a part of the DNA polymerase delta complex POLD4 contributes to polymerase and exonuclease activities required during cell division.
- Biological function summary
POLD4 plays an integral role in maintaining genomic stability through its function as part of the DNA polymerase delta complex. It is involved in synthesizing the lagging strand during DNA replication and facilitates repair through DNA mismatch repair processes. Its activity ensures accurate DNA synthesis thereby preventing mutations that might otherwise propagate during cell division. Interactions with proliferating cell nuclear antigen (PCNA) and other subunits of the polymerase delta complex POLD1 POLD2 and POLD3 are important for its function.
- Pathways
POLD4 participates in essential DNA replication and repair pathways. It plays a role in the replication fork by ensuring the fidelity of DNA synthesis coordinating with the proteins MSH2 and MLH1 in the mismatch repair pathway. Additionally POLD4's interaction with the replicative DNA helicase complex highlights its involvement in the initiation and elongation phases of DNA replication important for S-phase progression.
- Associated diseases and disorders
POLD4's involvement in DNA replication and repair processes links it to cancer progression. Mutations or dysregulation in POLD4 can lead to genomic instability often observed in various cancers due to defective mismatch repair. The connection between POLD4 and mismatch repair proteins like MSH2 is significant in cancers with microsatellite instability. Additionally POLD4's function is under investigation in relation to neurodegenerative disorders where DNA repair deficits contribute to disease pathology.
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SDS-PAGE - Recombinant Human POLD4 protein (ab171583)
15% SDS-PAGE analysis of ab171583 at 3ug.
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