Recombinant human Poliovirus Receptor/PVR protein (Active) is a Human Fragment protein, in the 21 to 343 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
W P P P G T G D V V V Q A P T Q V P G F L G D S V T L P C Y L Q V P N M E V T H V S Q L T W A R H G E S G S M A V F H Q T Q G P S Y S E S K R L E F V A A R L G A E L R N A S L R M F G L R V E D E G N Y T C L F V T F P Q G S R S V D I W L R V L A K P Q N T A E V Q K V Q L T G E P V P M A R C V S T G G R P P A Q I T W H S D L G G M P N T S Q V P G F L S G T V T V T S L W I L V P S S Q V D G K N V T C K V E H E S F E K P Q L L T V N L T V Y Y P P E V S I S G Y D N N W Y L G Q N E A T L T C D A R S N P E P T G Y N W S T T M G P L P P F A V A Q G A Q L L I R P V D K P I N T T L I C N V T N A L G A R Q A E L T V Q V K E G P P S E H S G I S R N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. (Microbial infection) Acts as a receptor for Pseudorabies virus. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.
Alternative names
CD155, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5
Recommended products
Recombinant human Poliovirus Receptor/PVR protein (Active) is a Human Fragment protein, in the 21 to 343 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Loaded Recombinant Human CD226 protein (ab206028) on Protein A Biosensor, can bind ab155723 with an affinity constant of 1.1 μM as determined in BLI assay.
Loaded Recombinant human TIGIT protein (ab223110) on Protein A Biosensor, can bind ab155723 with an affinity constant of 0.92 μM as determined in BLI assay.
- Accession
- P15151-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- W P P P G T G D V V V Q A P T Q V P G F L G D S V T L P C Y L Q V P N M E V T H V S Q L T W A R H G E S G S M A V F H Q T Q G P S Y S E S K R L E F V A A R L G A E L R N A S L R M F G L R V E D E G N Y T C L F V T F P Q G S R S V D I W L R V L A K P Q N T A E V Q K V Q L T G E P V P M A R C V S T G G R P P A Q I T W H S D L G G M P N T S Q V P G F L S G T V T V T S L W I L V P S S Q V D G K N V T C K V E H E S F E K P Q L L T V N L T V Y Y P P E V S I S G Y D N N W Y L G Q N E A T L T C D A R S N P E P T G Y N W S T T M G P L P P F A V A Q G A Q L L I R P V D K P I N T T L I C N V T N A L G A R Q A E L T V Q V K E G P P S E H S G I S R N
- Accession
- P15151
- Protein length
- Fragment
- Predicted molecular weight
- 35.9 kDa
- Amino acids
- 21 to 343
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
ab155723 was lyophilized from 0.22 µm filtered solution.
General info
- Function
Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.
- Sequence similarities
Belongs to the nectin family.
- Post-translational modifications
N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Poliovirus Receptor.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.
- Biological function summary
The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.
- Pathways
PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.
- Associated diseases and disorders
PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.
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4 product images
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Active) (ab155723)
Loaded Recombinant human TIGIT protein (Recombinant human TIGIT protein (Fc Chimera Active) ab223110) on Protein A Biosensor, can bind ab155723 with an affinity constant of 0.92 μM as determined in BLI assay.
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Active) (ab155723)
Loaded Recombinant Human CD226 protein (Recombinant Human CD226 protein (Fc Chimera) ab206028) on Protein A Biosensor, can bind ab155723 with an affinity constant of 1.1 μM as determined in BLI assay.
SDS-PAGE - Recombinant human Poliovirus Receptor/PVR protein (Active) (ab155723)
SDS-PAGE analysis of reduced ab155723 stained overnight with Coomassie Blue. The protein migrates as 55-65 kDa due to glycosylation.
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Active) (ab155723)
The purity of ab155723 was more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.
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