Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active)
- Bioactive
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Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) is a Human Fragment protein, in the 21 to 343 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.
View Alternative Names
CD155, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5
- FuncS
Unknown
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
FACS assay shows that recombinant Human CD155, Fc Tag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 µg/ml.
- FuncS
Supplier Data
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
Serial dilutions of Human TIGIT Neutralizing antibody were added into ab220561 : Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.06065 μg/mL (Routinely tested).
- FuncS
Unknown
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
Immobilized Human CD155, Fc Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 5-78 ng/mL.
- FuncS
Unknown
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
FACS analysis shows that the binding of Human CD155, Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 µg/ml. The IC50 is 0.09422 µg/ml.
- FuncS
Supplier Data
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
Loaded ab220561 on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e).
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (AB220561)
SDS-PAGE analysis of reduced ab220561 stained overnight with Coomassie Blue.
The protein migrates as 95-105 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.
Pathways
PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.
Specifications
Form
Lyophilized
General info
Function
Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors : CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.. (Microbial infection) Acts as a receptor for Pseudorabies virus.. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.
Sequence similarities
Belongs to the nectin family.
Post-translational modifications
N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4.. Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation.
Target data
Complementary Products
Product promise
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