Recombinant Human POLR2F protein (His tag N-Terminus)
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Recombinant Human POLR2F protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 127 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
POLRF, POLR2F, DNA-directed RNA polymerase II subunit F, RPABC14.4, RPB6 homolog, RPC15, RPB14.4
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human POLR2F protein (His tag N-Terminus) (AB180291)
15% SDS-PAGE (3μg)
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNA polymerase II mediates the transcription of DNA into precursor mRNA a critical step in gene expression. POLR2F is a core component of this multi-subunit complex alongside other proteins like POLR2A and POLR2B which form the catalytic center. The POLR2F-containing complex facilitates the engagement with DNA and progresses transcription elongation. Its interaction with various transcription factors and coactivators ensures the precise transcriptional regulation necessary for cellular functions.
Pathways
The POLR2F subunit plays a vital role in the RNA transcription pathway particularly in eukaryotic cells. It is involved in pathways such as the regulation of transcription and mRNA processing acting alongside proteins such as TFIIB and TFIIF. These proteins assist in initializing and stabilizing the transcription pre-initiation complex ensuring safe progression and termination of mRNA transcription from genomic DNA.
Specifications
Form
Liquid
Additional notes
purified by using conventional chromatography techniques.
General info
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPABC2 is part of the clamp element and together with parts of POLR2A/RPB1 and POLR2B/RPB2 forms a pocket to which the POLR2D/RPB4-POLR2G/RPB7 subcomplex binds.
Sequence similarities
Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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