Recombinant Human POLR2J3 protein is a Full Length protein, in the 1 to 115 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M N A P P A F E S F L L F E G E K I T I N K D T K V P N A C L F T I N K E D H T L G N I I K S Q L L K D P Q V L F A G Y K V P H P L E H K I I I R V Q T T P D Y S P Q E A F T N A I T D L I S E L S L L E E R F R T C L L P L R L L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Recommended products
Recombinant Human POLR2J3 protein is a Full Length protein, in the 1 to 115 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Animal free
- No
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M N A P P A F E S F L L F E G E K I T I N K D T K V P N A C L F T I N K E D H T L G N I I K S Q L L K D P Q V L F A G Y K V P H P L E H K I I I R V Q T T P D Y S P Q E A F T N A I T D L I S E L S L L E E R F R T C L L P L R L L P
- Protein length
- Full Length
- Predicted molecular weight
- 15.5 kDa
- Amino acids
- 1 to 115
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134600 is purified using conventional chromatography techniques.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLR2J3 also known as RNA polymerase II subunit J3 is a component of the RNA polymerase II (Pol II) complex which is responsible for synthesizing messenger RNA in eukaryotic cells. It has a molecular mass of approximately 13 kDa. This subunit is expressed mainly in various human tissues including liver kidney and skeletal muscle. Its expression levels can vary depending on the tissue type and cellular conditions.
- Biological function summary
RNA polymerase II acts as a central player in the transcription machinery. POLR2J3 forms an integral part of this complex playing a role in the assembly and stability of the whole machinery. The Pol II complex contains several other key subunits among them POLR2A and POLR2B which together function to unwind DNA polymerize RNA and ensure appropriate processing and modification of the nascent transcript. Changes in POLR2J3 expression may affect Pol II function and influence gene expression patterns in cells.
- Pathways
Transcription regulation and mRNA processing heavily involve the functioning of POLR2J3 within the Pol II complex. It supports the transcription of DNA to messenger RNA a critical step in the RNA polymerase enzyme pathway. POLR2J3 works closely with POLR2A among other subunits to coordinate this process ensuring the correct synthesis and modification of RNA. This protein's role within these pathways also connects it indirectly to signal transduction pathways that dictate cellular responses to different stimuli.
- Associated diseases and disorders
POLR2J3's role in transcription links it to potential involvement in cancer and neurodegenerative diseases. Errors in RNA polymerase II function which can arise from malfunctions within POLR2J3 have been observed in some cancer types due to defective cellular transcription processes. Furthermore alterations in transcription regulation possibly connected to POLR2J3 are implicated in neurological disorders where transcriptional dysregulation can lead to aberrant gene expression. Interactions between POLR2J3 and POLR2A during these processes suggest that both subunits might contribute to pathologies when their functions are compromised.
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1 product image
SDS-PAGE - Recombinant Human POLR2J3 protein (ab134600)
15% SDS-PAGE analysis of ab134600 (3μg).
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