Recombinant Human POLR2K protein is a Human Full Length protein, in the 1 to 58 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M D T Q K D V Q P P K Q Q P M I Y I C G E C H T E N E I K S R D P I R C R E C G Y R I M Y K K R T K R L V V F D A R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
Alternative names
ABC10-alpha, DNA-directed RNA polymerase II subunit K, RNA polymerase II 7.0 kDa subunit, RPB10alpha, RPB7.0, POLR2K
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Recombinant Human POLR2K protein is a Human Full Length protein, in the 1 to 58 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P53803-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M D T Q K D V Q P P K Q Q P M I Y I C G E C H T E N E I K S R D P I R C R E C G Y R I M Y K K R T K R L V V F D A R
- Accession
- P53803
- Protein length
- Full Length
- Predicted molecular weight
- 9.4 kDa
- Amino acids
- 1 to 58
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified using conventional chromatography techniques.
General info
- Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
- Sequence similarities
Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLR2K also known as Polymerase (RNA) II (DNA Directed) Polypeptide K is a subunit of the RNA polymerase II complex. This protein is involved in the transcription of DNA into mRNA. POLR2K has a molecular mass of approximately 7.52 kDa. It is expressed in various tissues throughout the body indicating its essential role in general cellular functions. The presence of POLR2K in different cell types emphasizes its importance in the transcription machinery.
- Biological function summary
POLR2K is part of the larger RNA polymerase II complex which is important for the synthesis of messenger RNA in eukaryotic cells. It functions in conjunction with other subunits to enable the initiation and elongation phases of transcription. POLR2K contributes to the accurate and efficient transcription of genetic information ensuring proper gene expression and cellular function. As a component of this complex POLR2K plays a significant role in the gene expression process.
- Pathways
The RNA polymerase II complex which includes POLR2K is an important player in the mRNA synthesis pathway. It is involved in the pathway for the synthesis of RNA from DNA templates often called the central dogma of molecular biology. POLR2K interacts with other subunits of RNA polymerase II such as RPB1 and RPB2 allowing for coordinated transcriptional regulation. This interaction underlines the integration of POLR2K within the transcriptional machinery.
- Associated diseases and disorders
Abnormal POLR2K function has been implicated in various pathological conditions. Dysregulation of the transcription machinery can contribute to cancer progression as evidence suggests involvement of POLR2K-related components. Its role in transcription aligns with disruptions in cellular homeostasis contributing to tumorigenesis. Additionally mutations affecting RNA polymerase II components including POLR2K can relate to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) highlighting the protein's role in maintaining neuronal function.
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1 product image
SDS-PAGE - Recombinant Human POLR2K protein (ab187471)
15% SDS-PAGE analysis of ab187471 (3μg).
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