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AB180315

Recombinant Human POLR3K protein - BSA and Azide free (His tag N-Terminus)

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Recombinant Human POLR3K protein - BSA and Azide free (His tag N-Terminus) is a Human Full Length protein, in the 1 to 108 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

RPC11, My010, POLR3K, DNA-directed RNA polymerase III subunit RPC10, RNA polymerase III subunit C10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RNA polymerase III subunit C11, RPC12.5, HsC11p, hRPC11

1 Images
SDS-PAGE - Recombinant Human POLR3K protein - BSA and Azide free (His tag N-Terminus) (AB180315)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human POLR3K protein - BSA and Azide free (His tag N-Terminus) (AB180315)

15% SDS-PAGE analysis of ab180315 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y2Y1

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD","proteinLength":"Full Length","predictedMolecularWeight":"14.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":108,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y2Y1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab180315 was purified by using conventional chromatography techniques.

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General info

Function

Core component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates (PubMed : 20413673, PubMed : 30584594, PubMed : 33335104, PubMed : 33558764, PubMed : 33558766, PubMed : 33674783, PubMed : 34675218). Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol III active site to constantly monitor transcription fidelity, cleaves mis-incorporated 5'-ribonucleotides and restarts the transcription process. Once Pol III reaches the poly(dT) termination signal, can induce Pol III clamp opening and transcription termination (By similarity) (PubMed : 33335104, PubMed : 33558764, PubMed : 33558766, PubMed : 33674783, PubMed : 34675218). Pol III plays an important role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed : 19609254, PubMed : 19631370).

Sequence similarities

Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Core component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates (PubMed : 20413673, PubMed : 30584594, PubMed : 33335104, PubMed : 33558764, PubMed : 33558766, PubMed : 33674783, PubMed : 34675218). Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol III active site to constantly monitor transcription fidelity, cleaves mis-incorporated 5'-ribonucleotides and restarts the transcription process. Once Pol III reaches the poly(dT) termination signal, can induce Pol III clamp opening and transcription termination (By similarity) (PubMed : 33335104, PubMed : 33558764, PubMed : 33558766, PubMed : 33674783, PubMed : 34675218). Pol III plays an important role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed : 19609254, PubMed : 19631370).
See full target information POLR3K
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