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Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) is a Human Fragment protein, in the 19 to 638 aa range, expressed in HEK 293, with >98% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

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Images

SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (AB174011), expandable thumbnail
  • SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (AB174011), expandable thumbnail

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 0.1 EU/µg
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
SDS-PAGE, ELISA, FuncS
Biologically active
Yes

Amino acid sequence

K S P I F G P E E V N S V E G N S V S I T C Y Y P P T S V N R H T R K Y W C R Q G A R G G C I T L I S S E G Y V S S K Y A G R A N L T N F P E N G T F V V N I A Q L S Q D D S G R Y K C G L G I N S R G L S F D V S L E V S Q G P G L L N D T K V Y T V D L G R T V T I N C P F K T E N A Q K R K S L Y K Q I G L Y P V L V I D S S G Y V N P N Y T G R I R L D I Q G T G Q L L F S V V I N Q L R L S D A G Q Y L C Q A G D D S N S N K K N A D L Q V L K P E P E L V Y E D L R G S V T F H C A L G P E V A N V A K F L C R Q S S G E N C D V V V N T L G K R A P A F E G R I L L N P Q D K D G S F S V V I T G L R K E D A G R Y L C G A H S D G Q L Q E G S P I Q A W Q L F V N E E S T I P R S P T V V K G V A G G S V A V L C P Y N R K E S K S I K Y W C L W E G A Q N G R C P L L V D S E G W V K A Q Y E G R L S L L E E P G N G T F T V I L N Q L T S R D A G F Y W C L T N G D T L W R T T V E I K I I E G E P N L K V P G N V T A V L G E T L K V P C H F P C K F S S Y E K Y W C K W N N T G C Q A L P S Q D E G P S K A F V N C D E N S R L V S L T L N L V T R A D E G W Y W C G V K Q G H F Y G E T A A V Y V A V E E R K A A G S R D V S L A K A D A A P D E K V L D S G F R E I E N K A I Q D P R L F A E E K A V A D T R D Q A D G S R A S V D S G S S E E Q G G S S R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed:12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed:16543244).

Alternative names

Recommended products

Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) is a Human Fragment protein, in the 19 to 638 aa range, expressed in HEK 293, with >98% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 0.1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, ELISA, FuncS
Biological activity
Measured by its binding ability in a functional ELISA.
Immobilized ab174011 at 2 μg/ml, the concentration of Human IgM that produces 50% of the optimal binding response is appoximately 20‑200 ng/ml.
Accession
P01833-1
Animal free
No
Carrier free
Yes
Species
Human
Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 100% PBS

Sequence info

Amino acid sequence

K S P I F G P E E V N S V E G N S V S I T C Y Y P P T S V N R H T R K Y W C R Q G A R G G C I T L I S S E G Y V S S K Y A G R A N L T N F P E N G T F V V N I A Q L S Q D D S G R Y K C G L G I N S R G L S F D V S L E V S Q G P G L L N D T K V Y T V D L G R T V T I N C P F K T E N A Q K R K S L Y K Q I G L Y P V L V I D S S G Y V N P N Y T G R I R L D I Q G T G Q L L F S V V I N Q L R L S D A G Q Y L C Q A G D D S N S N K K N A D L Q V L K P E P E L V Y E D L R G S V T F H C A L G P E V A N V A K F L C R Q S S G E N C D V V V N T L G K R A P A F E G R I L L N P Q D K D G S F S V V I T G L R K E D A G R Y L C G A H S D G Q L Q E G S P I Q A W Q L F V N E E S T I P R S P T V V K G V A G G S V A V L C P Y N R K E S K S I K Y W C L W E G A Q N G R C P L L V D S E G W V K A Q Y E G R L S L L E E P G N G T F T V I L N Q L T S R D A G F Y W C L T N G D T L W R T T V E I K I I E G E P N L K V P G N V T A V L G E T L K V P C H F P C K F S S Y E K Y W C K W N N T G C Q A L P S Q D E G P S K A F V N C D E N S R L V S L T L N L V T R A D E G W Y W C G V K Q G H F Y G E T A A V Y V A V E E R K A A G S R D V S L A K A D A A P D E K V L D S G F R E I E N K A I Q D P R L F A E E K A V A D T R D Q A D G S R A S V D S G S S E E Q G G S S R
Accession
P01833
Protein length
Fragment
Predicted molecular weight
68.7 kDa
Amino acids
19 to 638
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Lyophilized
Additional notes

ab174011 was lyophilized from 0.22 µm filtered solution.

General info

Function

Polymeric immunoglobulin receptor

Post-translational modifications

N-glycosylated. N-glycosylation is required for anchoring IgA molecules to mucus, but is not necessary for Ig binding.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Previously labelled as PIGR.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The polymeric immunoglobulin receptor often referred to as PIGR or the polymeric Ig receptor is a critical component in the immune system. This protein which has a mass of approximately 83 kDa is expressed primarily on the basolateral surface of epithelial cells. Common expression sites include the respiratory gastrointestinal and genital tracts. PIGR facilitates the transcytosis of polymeric immunoglobulins like IgA and IgM across epithelial cells ensuring that these antibodies reach mucosal sites where they perform functions.

Biological function summary

The polymeric immunoglobulin receptor plays an essential role in mucosal immunity by transporting IgA and IgM antibodies to mucosal surfaces. The PIGR is a part of a complex that includes the secretory component which protects immunoglobulins from proteolytic degradation. This mechanism is fundamental for safeguarding mucosal surfaces while allowing the immune system to respond swiftly to pathogens.

Pathways

The interaction of PIGR with polymeric immunoglobulins is an important event in the mucosal immune pathway. This pathway is important in maintaining mucosal homeostasis and pathogen defense. PIGR works closely with immunoglobulin proteins like IgA and IgM enabling their rapid and efficient transport across epithelial barriers. This ensures that the immune system rapidly addresses potential threats at mucosal sites.

Associated diseases and disorders

Defects in PIGR function can lead to conditions like IgA nephropathy and selective IgA deficiency. These disorders highlight the importance of the polymeric Ig receptor in immune health and suggest its role in maintaining immunoglobulin balance. There is evidence suggesting potential connections between PIGR dysfunction and the proteins involved in immune regulation which helps in understanding how PIGR contributes to disease pathology.

Product promise

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2 product images

  • SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011), expandable thumbnail

    SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011)

    Human pIgR (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

  • SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011), expandable thumbnail

    SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011)

    The purity of ab174011 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 85-95 kDa due to glycosylation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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