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AB53376

Recombinant Human PON1 protein

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Recombinant Human PON1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1

3 Images
Western blot - Recombinant Human PON1 protein (AB53376)
  • WB

Unknown

Western blot - Recombinant Human PON1 protein (AB53376)

All lanes:

Western blot - Anti-PON1 antibody (<a href='/en-us/products/primary-antibodies/pon1-antibody-ab99037'>ab99037</a>) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Western blot - Recombinant Human PON1 protein (ab53376) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 40 kDa

Observed band size: 39 kDa

true

Exposure time: 3min

Western blot - Recombinant Human PON1 protein (AB53376)
  • WB

Unknown

Western blot - Recombinant Human PON1 protein (AB53376)

All lanes:

Western blot - Anti-PON1 antibody [17A12] (<a href='/en-us/products/primary-antibodies/pon1-antibody-17a12-ab24261'>ab24261</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human PON1 protein (ab53376) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/500 dilution

Predicted band size: 40 kDa

true

Exposure time: 1min

SDS-PAGE - Recombinant Human PON1 protein (AB53376)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PON1 protein (AB53376)

SDS Page analysis of ab53376.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P27169

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.242% Tris

storage-buffer

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Recombinant Human CETP protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CETP protein (GST tag N-Terminus) (AB114408)

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Western blot - Recombinant Human RBP4 protein (Tag Free) (AB59967)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB126597

Anti-PON1 antibody [EPR2893]

RabMAb|Recombinant

Immunoprecipitation - Anti-PON1 antibody [EPR2893] (AB126597)

  • 0
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab53376 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/pon1-antibody-17a12-ab24261'>ab24261</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P27169","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

Sequence similarities

Belongs to the paraoxonase family.

Post-translational modifications

Glycosylated.. The signal sequence is not cleaved.. Present in two forms, form B contains a disulfide bond, form A does not.

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Product protocols

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Target data

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
See full target information PON1
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Product promise

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