Recombinant Human PON1 protein - BSA and Azide free is a Human Full Length protein, in the 1 to 355 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, MS.
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Key facts
- Purity
- >90% Densitometry
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
Amino acid sequence
M A K L I A L T L L G M G L A L F R N H Q S S Y Q T R L N A L R E V Q P V E L P N C N L V K G I E T G S E D L E I L P N G L A F I S S G L K Y P G I K S F N P N S P G K I L L M D L N E E D P T V L E L G I T G S K F D V S S F N P H G I S T F T D E D N A M Y L L V V N H P D A K S T V E L F K F Q E E E K S L L H L K T I R H K L L P N L N D I V A V G P E H F Y G T N D H Y F L D P Y L Q S W E M Y L G L A W S Y V V Y Y S P S E V R V V A E G F D F A N G I N I S P D G K Y V Y I A E L L A H K I H V Y E K H A N W T L T P L K S L D F N T L V D N I S V D P E T G D L W V G C H P N G M K I F F Y D S E N P P A S E V L R I Q N I L T E E P K V T Q V Y A E N G T V L Q G S T V A S V Y K G K L L I G T V F H K A L Y C E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Alternative names
PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1
Recommended products
Recombinant Human PON1 protein - BSA and Azide free is a Human Full Length protein, in the 1 to 355 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, MS.
Key facts
- Purity
- >90% Densitometry
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
- Accession
- P27169-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
- Storage buffer
Constituents: 99% Tris buffer, 0.29% Sodium chloride
Sequence info
Amino acid sequence
- M A K L I A L T L L G M G L A L F R N H Q S S Y Q T R L N A L R E V Q P V E L P N C N L V K G I E T G S E D L E I L P N G L A F I S S G L K Y P G I K S F N P N S P G K I L L M D L N E E D P T V L E L G I T G S K F D V S S F N P H G I S T F T D E D N A M Y L L V V N H P D A K S T V E L F K F Q E E E K S L L H L K T I R H K L L P N L N D I V A V G P E H F Y G T N D H Y F L D P Y L Q S W E M Y L G L A W S Y V V Y Y S P S E V R V V A E G F D F A N G I N I S P D G K Y V Y I A E L L A H K I H V Y E K H A N W T L T P L K S L D F N T L V D N I S V D P E T G D L W V G C H P N G M K I F F Y D S E N P P A S E V L R I Q N I L T E E P K V T Q V Y A E N G T V L Q G S T V A S V Y K G K L L I G T V F H K A L Y C E L
- Accession
- P27169
- Protein length
- Full Length
- Predicted molecular weight
- 40.68 kDa
- Amino acids
- 1 to 355
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purifed using Ni-NTA chromatography.
General info
- Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
- Sequence similarities
Belongs to the paraoxonase family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human PON1 protein - BSA and Azide free (ab175468)
12% SDS-PAGE analysis of ab175468
Lane 1: reduced and boiled sample (2.5 μg)
Lane 2: non-reduced and non-boiled sample (2.5 μg)
Downloads
Product protocols
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