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AB175468

Recombinant Human PON1 protein - BSA and Azide free

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Recombinant Human PON1 protein - BSA and Azide free is a Human Full Length protein, in the 1 to 355 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB, Mass Spec.

View Alternative Names

PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1

1 Images
SDS-PAGE - Recombinant Human PON1 protein - BSA and Azide free (AB175468)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PON1 protein - BSA and Azide free (AB175468)

12% SDS-PAGE analysis of ab175468

Lane 1 : reduced and boiled sample (2.5 μg)

Lane 2 : non-reduced and non-boiled sample (2.5 μg)

Key facts

Purity

>90% Densitometry

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

ELISA, Mass Spec, SDS-PAGE, WB

applications

Biologically active

No

Accession

P27169

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in 200 µL of water

Storage buffer

Constituents: 99% Tris buffer, 0.29% Sodium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB126597

Anti-PON1 antibody [EPR2893]

RabMAb|Recombinant

Western blot - Anti-PON1 antibody [EPR2893] (AB126597)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB59967

Recombinant Human RBP4 protein

Western blot - Recombinant Human RBP4 protein (AB59967)

  • 0
  • 0
Assay Kits

AB241044

Paraoxonase 1 Activity Assay Kit

Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCEL","proteinLength":"Full Length","predictedMolecularWeight":"40.68 kDa","actualMolecularWeight":null,"aminoAcidEnd":355,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P27169","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

Additional notes

Purifed using Ni-NTA chromatography.

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General info

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

Sequence similarities

Belongs to the paraoxonase family.

Post-translational modifications

Glycosylated.. The signal sequence is not cleaved.. Present in two forms, form B contains a disulfide bond, form A does not.

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Product protocols

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Target data

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
See full target information PON1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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