Recombinant Human PP-X protein (denatured) is a Human Full Length protein, in the 1 to 307 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A E I S D L D R Q I E Q L R R C E L I K E S E V K A L C A K A R E I L V E E S N V Q R V D S P V T V C G D I H G Q F Y D L K E L F R V G G D V P E T N Y L F M G D F V D R G F Y S V E T F L L L L A L K V R Y P D R I T L I R G N H E S R Q I T Q V Y G F Y D E C L R K Y G S V T V W R Y C T E I F D Y L S L S A I I D G K I F C V H G G L S P S I Q T L D Q I R T I D R K Q E V P H D G P M C D L L W S D P E D T T G W G V S P R G A G Y L F G S D V V A Q F N A A N D I D M I C R A H Q L V M E G Y K W H F N E T V L T V W S A P N Y C Y R C G N V A A I L E L D E H L Q K D F I I F E A A P Q E T R G I P S K K P V A D Y F L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Alternative names
PPP4, PPX, PPP4C, Serine/threonine-protein phosphatase 4 catalytic subunit, PP4C, Pp4, Protein phosphatase X, PP-X
Recommended products
Recombinant Human PP-X protein (denatured) is a Human Full Length protein, in the 1 to 307 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P60510-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A E I S D L D R Q I E Q L R R C E L I K E S E V K A L C A K A R E I L V E E S N V Q R V D S P V T V C G D I H G Q F Y D L K E L F R V G G D V P E T N Y L F M G D F V D R G F Y S V E T F L L L L A L K V R Y P D R I T L I R G N H E S R Q I T Q V Y G F Y D E C L R K Y G S V T V W R Y C T E I F D Y L S L S A I I D G K I F C V H G G L S P S I Q T L D Q I R T I D R K Q E V P H D G P M C D L L W S D P E D T T G W G V S P R G A G Y L F G S D V V A Q F N A A N D I D M I C R A H Q L V M E G Y K W H F N E T V L T V W S A P N Y C Y R C G N V A A I L E L D E H L Q K D F I I F E A A P Q E T R G I P S K K P V A D Y F L
- Accession
- P60510
- Protein length
- Full Length
- Predicted molecular weight
- 37.5 kDa
- Amino acids
- 1 to 307
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
- Sequence similarities
Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
- Post-translational modifications
Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human PP-X protein (denatured) (ab171584)
15% SDS-PAGE analysis of ab171584 at 3ug.
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