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AB184587

Recombinant Human PPIH protein

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Recombinant Human PPIH protein is a Human Full Length protein, in the 2 to 177 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB, Mass Spec.

View Alternative Names

CYP20, CYPH, PPIH, Peptidyl-prolyl cis-trans isomerase H, PPIase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, U-snRNP-associated cyclophilin SnuCyp-20, CypH, USA-CYP

1 Images
SDS-PAGE - Recombinant Human PPIH protein (AB184587)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PPIH protein (AB184587)

14 % SDS-PAGE separation of Human PPIH

MW : Molecular weight marker

Lane 1 : reduced and boiled sample, 2.5 μg
Lane 2 : non-reduced and non-boiled sample, 2.5 μg

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, ELISA, WB, Mass Spec

applications

Biologically active

No

Accession

O43447

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml.

Storage buffer

Constituents: PBS

storage-buffer

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Primary Antibodies

AB151246

Anti-PPIH antibody

Immunocytochemistry/ Immunofluorescence - Anti-PPIH antibody (AB151246)

  • 0
  • 0
Proteins & Peptides

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKHHHHHHASAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM","proteinLength":"Full Length","predictedMolecularWeight":"20.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":177,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43447","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The peptidyl-prolyl cis-trans isomerase H (PPIH) also known as cyclophilin H is involved in protein folding and assembly. PPIH has a molecular weight of approximately 18 kDa. This protein expresses widely in human tissues with a notable presence in the nucleus. As an isomerase PPIH accelerates the cis-trans isomerization of prolyl bonds an important step in protein folding influencing protein conformation and function.
Biological function summary

PPIH plays a significant role in pre-mRNA splicing. It acts as a component of the spliceosome a critical molecular machinery for removing introns from pre-mRNA. Through its action within the spliceosome PPIH helps regulate the maturation of mRNA and by extension protein synthesis. Its interactions ensure the stability and functional configuration of the spliceosomal complex.

Pathways

PPIH participates in mRNA processing and splicing pathways. It engages in the spliceosomal pathway aligning closely with proteins like SMN1 and the rest of the spliceosome complex which are involved in RNA processing and gene expression regulation. The interplay of PPIH in these pathways highlights its essential role in gene expression and cellular growth functions.

Research connects PPIH with certain neurodegenerative disorders and cancer. Abnormalities in PPIH function or expression might influence diseases like spinal muscular atrophy due to its relationship with SMN1. Additionally dysregulation of PPIH-connected pathways could contribute to oncogenesis supporting its significance in cancer research. Scientists continue exploring PPIH to develop targeted therapies for these conditions.
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Specifications

Form

Lyophilized

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General info

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed : 20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.

Sequence similarities

Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.

Subcellular localisation

Nucleus speckle

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Product protocols

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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed : 20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
See full target information PPIH
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Product promise

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