Recombinant human PPIH protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A V A N S S P V N P V V F F D V S I G G Q E V G R M K I E L F A D V V P K T A E N F R Q F C T G E F R K D G V P I G Y K G S T F H R V I K D F M I Q G G D F V N G D G T G V A S I Y R G P F A D E N F K L R H S A P G L L S M A N S G P S T N G C Q F F I T C S K C D W L D G K H V V F G K I I D G L L V M R K I E N V P T G P N N K P K L P V V I S Q C G E M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
Alternative names
CYP20, CYPH, PPIH, Peptidyl-prolyl cis-trans isomerase H, PPIase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, U-snRNP-associated cyclophilin SnuCyp-20, CypH, USA-CYP
Recommended products
Recombinant human PPIH protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 220nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.
- Accession
- O43447-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M A V A N S S P V N P V V F F D V S I G G Q E V G R M K I E L F A D V V P K T A E N F R Q F C T G E F R K D G V P I G Y K G S T F H R V I K D F M I Q G G D F V N G D G T G V A S I Y R G P F A D E N F K L R H S A P G L L S M A N S G P S T N G C Q F F I T C S K C D W L D G K H V V F G K I I D G L L V M R K I E N V P T G P N N K P K L P V V I S Q C G E M
- Accession
- O43447
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab78874 is purified using conventional chromatography techniques.
General info
- Function
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
- Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.
- Subcellular localisation
- Nucleus speckle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The peptidyl-prolyl cis-trans isomerase H (PPIH) also known as cyclophilin H is involved in protein folding and assembly. PPIH has a molecular weight of approximately 18 kDa. This protein expresses widely in human tissues with a notable presence in the nucleus. As an isomerase PPIH accelerates the cis-trans isomerization of prolyl bonds an important step in protein folding influencing protein conformation and function.
- Biological function summary
PPIH plays a significant role in pre-mRNA splicing. It acts as a component of the spliceosome a critical molecular machinery for removing introns from pre-mRNA. Through its action within the spliceosome PPIH helps regulate the maturation of mRNA and by extension protein synthesis. Its interactions ensure the stability and functional configuration of the spliceosomal complex.
- Pathways
PPIH participates in mRNA processing and splicing pathways. It engages in the spliceosomal pathway aligning closely with proteins like SMN1 and the rest of the spliceosome complex which are involved in RNA processing and gene expression regulation. The interplay of PPIH in these pathways highlights its essential role in gene expression and cellular growth functions.
- Associated diseases and disorders
Research connects PPIH with certain neurodegenerative disorders and cancer. Abnormalities in PPIH function or expression might influence diseases like spinal muscular atrophy due to its relationship with SMN1. Additionally dysregulation of PPIH-connected pathways could contribute to oncogenesis supporting its significance in cancer research. Scientists continue exploring PPIH to develop targeted therapies for these conditions.
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1 product image
SDS-PAGE - Recombinant human PPIH protein (ab78874)
15% SDS-PAGE showing ab78874 at approximately 19kDa (3μg).
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