Recombinant human PPIL3 protein is a Human Full Length protein, in the 1 to 161 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, FuncS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS, FuncS
- Biologically active
Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S V T L H T D V G D I K I E V F C E R T P K T C E N F L A L C A S N Y Y N G C I F H R N I K G F M V Q T G D P T G T G R G G N S I W G K K F E D E Y S E Y L K H N V R G V V S M A N N G P N T N G S Q F F I T Y G K Q P H L D M K Y T V F G K V I D G L E T L D E L E K L P V N E K T Y R P L N D V H I K D I T I H A N P F A Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
Alternative names
Peptidyl-prolyl cis-trans isomerase-like 3, PPIase, Cyclophilin J, Cyclophilin-like protein PPIL3, Rotamase PPIL3, CyPJ, PPIL3
Recommended products
Recombinant human PPIL3 protein is a Human Full Length protein, in the 1 to 161 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, FuncS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS, FuncS
- Biologically active
Yes
- Biological activity
Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.
- Accession
- Q9H2H8-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S V T L H T D V G D I K I E V F C E R T P K T C E N F L A L C A S N Y Y N G C I F H R N I K G F M V Q T G D P T G T G R G G N S I W G K K F E D E Y S E Y L K H N V R G V V S M A N N G P N T N G S Q F F I T Y G K Q P H L D M K Y T V F G K V I D G L E T L D E L E K L P V N E K T Y R P L N D V H I K D I T I H A N P F A Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
20.3 kDa
- Amino acids
1 to 161
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab113119 was purified using conventional chromatography.
General info
- Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
- Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PPIL3 or Peptidylprolyl isomerase (cyclophilin)-like 3 is an enzyme that assists in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It weighs approximately 52 kDa. PPIL3 expression can be found in various tissues including liver and thymus. The protein acts as a member of the cyclophilin family which is known for its role in protein folding and immunosuppression.
- Biological function summary
The function of PPIL3 includes its contribution to the assembly of spliceosomal complexes. It interacts with other proteins to aid the splicing of pre-mRNA therefore influencing gene expression regulation. Though PPIL3 operates independently it often localizes within multiprotein complexes involved in RNA processing. Its role in the spliceosome highlights its importance in the accurate and efficient editing of RNA transcripts.
- Pathways
PPIL3 participates in the RNA splicing and gene expression pathways. Within these pathways PPIL3 shows interactions with the spliceosomal proteins such as U2 small nuclear ribonucleoprotein highlighting its contribution to mRNA splicing regulation. It involves itself in adjusting the splicing machinery's fine-tuning ensuring correct mRNA processing essential for cellular functions.
- Associated diseases and disorders
PPIL3 has links to conditions such as cancer and neurodegeneration. Its role in RNA splicing means that any dysfunction resulting in spliceosomal assembly errors can contribute to diseases like cancer where incorrect mRNA splicing leads to aberrant gene expression. PPIL3 is also associated with cyclophilin B through their overlapping functions in protein folding and transport which can become impaired in neurodegenerative disorders.
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1 product image
SDS-PAGE - Recombinant human PPIL3 protein (ab113119)
15% SDS-PAGE analysis of ab113119 (3μg)
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