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AB113119

Recombinant human PPIL3 protein

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Recombinant human PPIL3 protein is a Human Full Length protein, in the 1 to 161 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase-like 3, PPIase, Cyclophilin J, Cyclophilin-like protein PPIL3, Rotamase PPIL3, CyPJ, PPIL3

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SDS-PAGE - Recombinant human PPIL3 protein (AB113119)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPIL3 protein (AB113119)

15% SDS-PAGE analysis of ab113119 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 μmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.

Accession

Q9H2H8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSVTLHTDVGDIKIEVFCERTPKTCENFLALCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKKFEDEYSEYLKHNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKTYRPLNDVHIKDITIHANPFAQ","proteinLength":"Full Length","predictedMolecularWeight":"20.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":161,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H2H8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPIL3 or Peptidylprolyl isomerase (cyclophilin)-like 3 is an enzyme that assists in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It weighs approximately 52 kDa. PPIL3 expression can be found in various tissues including liver and thymus. The protein acts as a member of the cyclophilin family which is known for its role in protein folding and immunosuppression.
Biological function summary

The function of PPIL3 includes its contribution to the assembly of spliceosomal complexes. It interacts with other proteins to aid the splicing of pre-mRNA therefore influencing gene expression regulation. Though PPIL3 operates independently it often localizes within multiprotein complexes involved in RNA processing. Its role in the spliceosome highlights its importance in the accurate and efficient editing of RNA transcripts.

Pathways

PPIL3 participates in the RNA splicing and gene expression pathways. Within these pathways PPIL3 shows interactions with the spliceosomal proteins such as U2 small nuclear ribonucleoprotein highlighting its contribution to mRNA splicing regulation. It involves itself in adjusting the splicing machinery's fine-tuning ensuring correct mRNA processing essential for cellular functions.

PPIL3 has links to conditions such as cancer and neurodegeneration. Its role in RNA splicing means that any dysfunction resulting in spliceosomal assembly errors can contribute to diseases like cancer where incorrect mRNA splicing leads to aberrant gene expression. PPIL3 is also associated with cyclophilin B through their overlapping functions in protein folding and transport which can become impaired in neurodegenerative disorders.
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Specifications

Form

Liquid

Additional notes

ab113119 was purified using conventional chromatography.

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General info

Function

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

Sequence similarities

Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.

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Product protocols

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Target data

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
See full target information PPIL3
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