JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101207

Recombinant human PPIL4 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PPIL4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 512 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase-like 4, PPIase, Cyclophilin-like protein PPIL4, Rotamase PPIL4, PPIL4

1 Images
SDS-PAGE - Recombinant human PPIL4 protein (His tag N-Terminus) (AB101207)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPIL4 protein (His tag N-Terminus) (AB101207)

ab101207 (3 μg) analysed by 15% SDS PAGE.
Molecular size on SDS-PAGE will appear higher than the predicted molecular weight of 59.4 kDa.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 μmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.

Accession

Q8WUA2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAVLLETTLGDVVIDLYTEERPRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTGDPTGTGRGGESIFGQLYGDQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDGVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPEPTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDLPDADIKPPENVLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIEFEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDKVKPKQDTKYDLILDEQAEDSKSSHSHTSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSPKKSKDKEKSKYR","proteinLength":"Full Length","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":512,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8WUA2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPIL4 also known as Peptidylprolyl Isomerase Like 4 is a member of the cyclophilin family of proteins. It exhibits peptidyl-prolyl cis-trans isomerase activity facilitating the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The protein has a molecular weight of around 56 kDa and is ubiquitously expressed in various tissues throughout the body.
Biological function summary

PPIL4 participates in protein folding and assembly and it plays a role in maintaining cellular functions. This protein is part of complexes involved in RNA processing and mRNA splicing within the cell. It may interact with other spliceosomal components indicating its involvement in the regulation of gene expression through alternative splicing.

Pathways

PPIL4 is involved in the regulation of mRNA splicing influencing the expression of genes at the post-transcriptional level. It is connected to the spliceosome pathway where it associates with proteins like SNRNP200 and other spliceosomal proteins to modulate pre-mRNA splicing. This connection aids in the precise control of gene expression necessary for various cellular processes.

Altered PPIL4 function has implications in certain cancers and neurodegenerative diseases. Changes in PPIL4 expression may link to the disruption of normal splicing activities contributing to malignancies like adenocarcinomas. The protein's association with complex components like SNRNP200 could play a role in these diseases by affecting RNA processing stability and fidelity which are critical for maintaining normal cellular activities.
style
left-column

Specifications

Form

Liquid

Additional notes

ab101207 was purified using conventional chromatography and is >85 % pure by SDS-PAGE.

style
left-column

General info

Function

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity).

Sequence similarities

Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity).
See full target information PPIL4
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB123160

Recombinant Human TICAM2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TICAM2 protein (His tag N-Terminus) (AB123160)

  • 0
  • 0
Proteins & Peptides

AB92433

Recombinant human HSPA2 protein

Western blot - Recombinant human HSPA2 protein (AB92433)

  • 0
  • 0
Proteins & Peptides

AB316636

Recombinant Human BMP2 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human BMP2 Protein Standard (His tag) (AB316636)

  • 0
  • 0
Proteins & Peptides

AB186986

Recombinant Human PIN4 protein

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com