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AB171704

Recombinant Human PPM1F/POPX2 protein (His tag N-Terminus)

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Recombinant Human PPM1F/POPX2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA0015, POPX2, PPM1F, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase, hFem-2

1 Images
SDS-PAGE - Recombinant Human PPM1F/POPX2 protein (His tag N-Terminus) (AB171704)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PPM1F/POPX2 protein (His tag N-Terminus) (AB171704)

15% SDS-PAGE analysis of ab171704 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P49593

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PPM1F
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSSGAPQKSSPMASGAEETPGFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAAALAHEAVSQLLQTDLSEFRKLPREEEEEEEDDDEEEKAPVTLLDAQSLAQSFFNRLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLPSFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQPELPTDPEGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQVILVQQGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRALTGSEDYLLLACDGFFDVVPHQEVVGLVQSHLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELLEGGNQGEGDPQAEGRRQDLPSSLPEPETQAPPRS","proteinLength":"Full Length","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":454,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49593","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1F also known as POPX2 is a protein phosphatase that belongs to the PP2C family. It functions as a serine/threonine phosphatase and has a molecular mass of approximately 48 kDa. PPM1F is expressed across various tissues including the brain muscle and liver. It plays a role in dephosphorylating proteins involved in numerous cellular processes. By regulating phosphorylation status PPM1F impacts cellular activities such as cell cycle progress and apoptotic pathways.
Biological function summary

This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.

Pathways

Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.

Alterations in PPM1F function have correlations with cancer and neurodegenerative diseases. In cancer PPM1F's dephosphorylation roles impact pathways relevant to cell proliferation and apoptosis contributing to tumor progression. Abnormal regulation of PPM1F has also been observed in certain neurodegenerative disorders where its interactions with proteins such as MAPKs affect neuronal survival and distress responses. The link between PPM1F and these conditions highlights its potential as a therapeutic target in disease management.
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Specifications

Form

Liquid

Additional notes

ab171704 is purified using conventional chromatography techniques.

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General info

Function

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Sequence similarities

Belongs to the PP2C family.

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Product protocols

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Target data

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
See full target information PPM1F
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Complementary Products

Primary Antibodies

AB200394

Anti-PPM1F/POPX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)

  • 0
  • 0
Primary Antibodies

AB179826

Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

  • 0
  • 0
Assay Kits

AB283990

Human PPM1F/POPX2 ELISA Kit

Sandwich ELISA - Human PPM1F/POPX2 ELISA Kit (AB283990)

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  • 0
Cell Lines & Lysates

AB266176

Human PPM1F (POPX2) knockout HEK-293T cell line

Sanger Sequencing - Human PPM1F (POPX2) knockout HEK-293T cell line (AB266176)

  • 0
  • 0

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