JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB128551

Recombinant human PPP1CB

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant human PPP1CB is a Human Full Length protein, in the 1 to 327 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, Phosphatase Activity, FuncS.

View Alternative Names

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

4 Images
Phosphatase Activity - Recombinant human PPP1CB (AB128551)
  • Phosphatase Activity

Unknown

Phosphatase Activity - Recombinant human PPP1CB (AB128551)

Phosphatase activity demonstrating specific activity of ab128551.

Functional Studies - Recombinant human PPP1CB (AB128551)
  • FuncS

Unknown

Functional Studies - Recombinant human PPP1CB (AB128551)

The specific activity of PPP1CB (ab128551) was determined to be 313 nmol/min/mg as per activity assay protocol

Western blot - Recombinant human PPP1CB (AB128551)
  • WB

Unknown

Western blot - Recombinant human PPP1CB (AB128551)

All lanes:

Western blot - Recombinant human PPP1CB (ab128551)

false

SDS-PAGE - Recombinant human PPP1CB (AB128551)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPP1CB (AB128551)

SDS PAGE analysis of ab128551

Key facts

Purity

>70% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, Phosphatase Activity, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab128551 was determined to be 272 nmol/min/mg

Accession

P62140

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.02% PMSF, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB128559

Recombinant human PPP1A/PPP1CA protein

Functional Studies - Recombinant human PPP1A/PPP1CA protein (AB128559)

  • 0
  • 0
Proteins & Peptides

AB159173

Recombinant Human PPP2R5A protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PPP2R5A protein (GST tag N-Terminus) (AB159173)

  • 0
  • 0
Primary Antibodies

AB53315

Anti-PPP1CB antibody [EP1804Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] (AB53315)

  • 5
  • 2
Proteins & Peptides

AB290069

Recombinant Human PP1A Protein

Mass Spectrometry - Recombinant Human PP1A Protein (AB290069)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Phosphatase Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Centrifuge before aliquoting into smaller quantities. This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB
style
left-column

Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P62140","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.
Biological function summary

PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.

Pathways

PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.

PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).
See full target information PPP1CB
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 129:1340-54 PubMed26872783

2016

PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Rogers,Dirk Fey,Rachael A McCloy,Benjamin L Parker,Nicholas J Mitchell,Richard J Payne,Roger J Daly,David E James,C Elizabeth Caldon,D Neil Watkins,David R Croucher,Andrew Burgess
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com