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AB171709

Recombinant Human PPP1R3B protein (denatured)

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Recombinant Human PPP1R3B protein (denatured) is a Human Full Length protein, in the 1 to 285 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

PPP1R4, PPP1R3B, Protein phosphatase 1 regulatory subunit 3B, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL, Protein phosphatase 1 regulatory subunit 4, Protein phosphatase 1 subunit GL, PP1 subunit R4, PTG

1 Images
SDS-PAGE - Recombinant Human PPP1R3B protein (denatured) (AB171709)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PPP1R3B protein (denatured) (AB171709)

15% SDS-PAGE analysis of ab171709 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q86XI6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMMAVDIEYRYNCMAPSLRQERFAFKISPKPSKPLRPCIQLSSKNEASGMVAPAVQEKKVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSYERMEFAVYYECNGQTYWDSNRGKNYRIIRAELKSTQGMTKPHSGPDLGISFDQFGSPRCSYGLFPEWPSYLGYEKLGPYY","proteinLength":"Full Length","predictedMolecularWeight":"35.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":285,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q86XI6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R3B also known as Protein Phosphatase 1 Regulatory Subunit 3B plays a mechanical role in glycogen metabolism. The protein affects the activity of glycogen synthase and glycogen phosphorylase by regulating the substrate specificity of Protein Phosphatase 1 (PP1) its enzymatic partner. PPP1R3B has a mass of approximately 33 kDa and its expression is primarily noted in liver and skeletal muscle tissues. The localization of PPP1R3B to these tissues suggests a specialized role in energy storage and regulation.
Biological function summary

Protein Phosphatase 1 Regulatory Subunit 3B influences glycogen synthesis and degradation. As a part of the PP1 holoenzyme complex PPP1R3B modulates the dephosphorylation of target proteins which controls glycogen metabolism. This regulation enables cells to respond to changes in energy demands effectively. PPP1R3B's interaction with other subunits in the PP1 complex allows for dynamic control of the metabolic processes impacting overall energy homeostasis.

Pathways

Regulation of glycogen metabolism involves several important steps where PPP1R3B integrates into the glycogen biosynthetic pathway and the insulin signaling pathway. By forming associations with other proteins such as glycogen synthase it exerts influence on how glycogen is synthesized and mobilized. In response to signals from upstream kinases such as Akt PPP1R3B works with PP1 to ensure the precise modulation of phosphorylation states that dictate glycogen synthase activity.

Irregularities in PPP1R3B functionality connect to conditions like type 2 diabetes and glycogen storage disease. Disruptions in its normal operation can result in improper glycogen mobilization and storage influencing blood glucose levels. The protein interacts with other regulatory proteins like the PP1 complex and AMPK which are involved in pathways linked to insulin sensitivity and energy balance. Understanding PPP1R3B's role in these disorders could provide insights for therapeutic strategies to manage metabolic diseases.
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Specifications

Form

Liquid

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General info

Function

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).

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Product protocols

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Target data

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).
See full target information PPP1R3B
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