Recombinant Human PPP2CB protein
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Recombinant Human PPP2CB protein is a Human Full Length protein, in the 1 to 309 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB.
View Alternative Names
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-beta, PPP2CB
- WB
Lab
Western blot - Recombinant Human PPP2CB protein (AB128562)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] (<a href='/en-us/products/primary-antibodies/pp2a-alpha-beta-antibody-epr11787b-ab168350'>ab168350</a>) at 1/20000 dilution
Lane 1:
Western blot - Recombinant Human PPP2CB protein (ab128562) at 0.015 µg
Lane 2:
Recombinant human PP2A-alpha protein (<a href='/en-us/products/unavailable/recombinant-human-pp2a-alpha-protein-active-ab128557'>ab128557</a>) at 0.015 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 59 kDa
false
Exposure time: 15s
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human PPP2CB protein (AB128562)
SDS-PAGE showing ab128562 at approximately 59 kDa
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein phosphatase activity of PPP2CB influences many cellular processes. It is a vital part of the protein phosphatase 2 (PP2A) complex which is a major serine/threonine phosphatase in cells. Through its involvement in the PP2A complex PPP2CB regulates cellular processes like cell growth division and apoptosis. The PP2A complex of which PPP2CB is a component remains essential to signaling pathways contributing to the dynamic behavior of cellular phosphorylation-dephosphorylation cycles.
Pathways
Protein phosphatase 2's function aligns PP2CB with key signaling routes such as the MAPK and Wnt pathways. In the MAPK pathway PP2A complex dephosphorylates specific kinases which controls cellular responses to external stimuli. Within the Wnt pathway the regulatory actions of PP2A maintain the appropriate level of pathway activity. The roles of PPP2CB in these pathways connect it to other related proteins like B56 family regulatory subunits in signal transduction networks.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (Probable). PP2A can modulate the activity of phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational modifications
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.. May be monoubiquitinated by NOSIP.
Subcellular localisation
Nucleus
Target data
Complementary Products
![Western blot - Anti-PPP2CB antibody [EPR11786(B)] (AB168371)](https://content.abcam.com/products/images/ppp2cb-antibody-epr11786b-ab168371--western-blot-img107271.jpg)
Product promise
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