Recombinant human PPP2R1A + PP2A alpha protein (Active) is a Human Full Length protein, in the 1 to 309 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M D E K V F T K E L D Q W I E Q L N E C K Q L S E S Q V K S L C E K A K E I L T K E S N V Q E V R C P V T V C G D V H G Q F H D L M E L F R I G G K S P D T N Y L F M G D Y V D R G Y Y S V E T V T L L V A L K V R Y R E R I T I L R G N H E S R Q I T Q V Y G F Y D E C L R K Y G N A N V W K Y F T D L F D Y L P L T A L V D G Q I F C L H G G L S P S I D T L D H I R A L D R L Q E V P H E G P M C D L L W S D P D D R G G W G I S P R G A G Y T F G Q D I S E T F N H A N G L T L V S R A H Q L V M E G Y N W C H D R N V V T I F S A P N Y C Y R C G N Q A A I M E L D D T L K Y S F L Q F D P A P R R G E P H V T R R T P D Y F L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753, PubMed:33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed:28982702).
Additional Targets
PPP2CA, PPP2CA
Alternative names
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A
Recommended products
Recombinant human PPP2R1A + PP2A alpha protein (Active) is a Human Full Length protein, in the 1 to 309 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).
- Accession
- P30153-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 1.05% MOPS, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence 1
- M D E K V F T K E L D Q W I E Q L N E C K Q L S E S Q V K S L C E K A K E I L T K E S N V Q E V R C P V T V C G D V H G Q F H D L M E L F R I G G K S P D T N Y L F M G D Y V D R G Y Y S V E T V T L L V A L K V R Y R E R I T I L R G N H E S R Q I T Q V Y G F Y D E C L R K Y G N A N V W K Y F T D L F D Y L P L T A L V D G Q I F C L H G G L S P S I D T L D H I R A L D R L Q E V P H E G P M C D L L W S D P D D R G G W G I S P R G A G Y T F G Q D I S E T F N H A N G L T L V S R A H Q L V M E G Y N W C H D R N V V T I F S A P N Y C Y R C G N Q A A I M E L D D T L K Y S F L Q F D P A P R R G E P H V T R R T P D Y F L
- Accession
- P67775
- Protein length
- Full Length
- Amino acids
- 1 to 309
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- Accession
- P30153
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753, PubMed:33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed:28982702).
- Sequence similarities
Belongs to the phosphatase 2A regulatory subunit A family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Proteins co-expressed.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PPP2R1A also referred to as PP2A alpha is a regulatory subunit of the protein phosphatase 2A (PP2A) complex. The protein has a mass of approximately 65 kDa. It functions mechanically as a scaffold subunit within the PP2A holoenzyme serving to coordinate the interaction between catalytic and regulatory subunits of the complex. This protein is widely expressed across various tissues which reflects its role in numerous cellular processes.
- Biological function summary
The activity of PPP2R1A is essential for the dephosphorylation of serine/threonine residues on proteins allowing significant regulation of various cellular functions. The protein is an important part of the PP2A complex a heterotrimer that consists of structural (A) catalytic (C) and regulatory (B) subunits. This complex contributes to the control of cell growth and division by maintaining appropriate levels of phosphorylation within the cell impacting various signaling pathways.
- Pathways
The functional activity of PPP2R1A integrates into critical pathways like the MAPK signaling pathway and cell cycle regulation. It modulates the activities of kinases and other proteins such as AKT and MEK which are critical to these pathways and are important in processes like cell proliferation and apoptosis. PPP2R1A's regulatory role ensures proper signal transduction within these pathways for maintaining cellular homeostasis.
- Associated diseases and disorders
The malfunction or mutation of PPP2R1A has been linked to cancers such as lung and colorectal cancer. Aberrant signaling within the pathways it regulates can lead to unregulated cell growth and contribute to tumorigenesis. Furthermore the protein's dysfunction can be associated with disorders connected to AKT signaling dysregulation reinforcing the importance of PPP2R1A's role in maintaining cellular structure and function.
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2 product images
Functional Studies - Recombinant human PPP2R1A + PP2A alpha protein (Active) (ab268879)
The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).
SDS-PAGE - Recombinant human PPP2R1A + PP2A alpha protein (Active) (ab268879)
SDS-PAGE analysis of ab268879.
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