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AB207146

Recombinant Human PPP2R1A protein

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Recombinant Human PPP2R1A protein is a Human Full Length protein, in the 1 to 589 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A

1 Images
SDS-PAGE - Recombinant Human PPP2R1A protein (AB207146)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PPP2R1A protein (AB207146)

15% SDS-PAGE analysis of 3 μg ab207146.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P30153

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA","proteinLength":"Full Length","predictedMolecularWeight":"67.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":589,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30153","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 scaffold subunit A alpha isoform (PPP2R1A) functions as an important regulatory component of the serine/threonine phosphatase PP2A complex. Alternate names for PPP2R1A include PR65A and Aalpha. This protein has a mass of approximately 65 kDa and is widely expressed in many tissues including the brain heart and lungs. It serves as a scaffold for regulatory and catalytic subunits facilitating their assembly into the active enzyme complex.
Biological function summary

The PP2A complex plays a role in cell cycle control apoptosis and signal transduction. PPP2R1A forms a dimer with one catalytic and one regulatory subunit. This arrangement influences various cell signaling processes controlling cell growth and division. Through its position in the PP2A complex PPP2R1A moderates phosphorylation status of target proteins impacting diverse cellular activities.

Pathways

PPP2R1A participates in pathways like the Wnt signaling and the MAPK pathways acting as a brake on kinase-driven signaling. In the Wnt signaling pathway PP2A mediates beta-catenin degradation which influences gene expression related to cell growth. Within the MAPK pathway PPP2R1A interacts with kinases to modulate cellular responses to growth factors. Proteins such as AXIN1 in the Wnt signaling context are associated with the regulatory effects mediated by PPP2R1A.

Alterations in PPP2R1A have been implicated in cancers like ovarian and uterine serous carcinoma. Mutations within this gene can disrupt PP2A activity leading to unchecked cell proliferation. Associations with beta-catenin highlight a significant role in tumorigenesis. In these contexts PPP2R1A's relationship with proteins such as beta-catenin and other components of the Wnt pathway indicates its importance in maintaining cellular homeostasis and its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab207146 was purified using conventional chromatography techniques.

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General info

Function

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed : 15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed : 16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753, PubMed : 33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed : 28982702).

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit A family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed : 15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed : 16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753, PubMed : 33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed : 28982702).
See full target information Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
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