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AB159172

Recombinant Human PPP2R2A protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human PPP2R2A protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 447 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, B55, PPP2R2A

1 Images
SDS-PAGE - Recombinant Human PPP2R2A protein (GST tag N-Terminus) (AB159172)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PPP2R2A protein (GST tag N-Terminus) (AB159172)

ab159172 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P63151

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB313413

Anti-PPP2R2A antibody [RP23040118]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R2A Antibody [RP23040118] (AB313413)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":447,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P63151","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein phosphatase 2 regulatory subunit B alpha (PPP2R2A) also known as B55α is a regulatory component of the protein phosphatase 2 (PP2A) complex. It is a 55 kDa subunit that modulates PP2A activity and substrate specificity. PPP2R2A is widely expressed in various human tissues with high levels in the brain heart and skeletal muscle. This regulatory subunit helps control the dephosphorylation of serine/threonine residues on target proteins playing an important role in cellular processes by directing the substrate selection of the PP2A complex.
Biological function summary

PPP2R2A influences several cellular processes through its integration into the PP2A holoenzyme complex. It directs the dephosphorylation of proteins like MAP kinases which are important for signal transduction. By associating with the catalytic and scaffolding subunits of PP2A PPP2R2A modulates cell cycle progression apoptosis and DNA replication. Its involvement in these processes highlights its importance in maintaining cellular homeostasis and function.

Pathways

PPP2R2A is integral to the MAPK/ERK and PI3K/AKT signaling pathways. It regulates the activity of several kinases and phosphatases in these pathways influencing cell growth and survival. In the MAPK/ERK pathway PPP2R2A interacts with proteins like MEK and ERK while in the PI3K/AKT pathway it can connect with the phosphatase PTEN highlighting its regulatory roles in critical cellular signaling networks.

PPP2R2A has been associated with cancer and neurodegenerative diseases. Its dysregulation can lead to aberrant cell proliferation as often seen in certain cancers. For instance altered expression of PPP2R2A has been noted in prostate cancer. In neurodegenerative conditions like Alzheimer's disease PPP2R2A interacts with tau protein affecting tau phosphorylation and potentially contributing to disease progression. The involvement of PPP2R2A in these diseases highlights its relevance as a potential therapeutic target.
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Specifications

Form

Liquid

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General info

Function

Substrate-recognition subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit (PubMed : 1849734, PubMed : 33108758). Involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67 (By similarity). Essential for serine/threonine-protein phosphatase 2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed : 33108758).

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B family.

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Product protocols

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Target data

Substrate-recognition subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit (PubMed : 1849734, PubMed : 33108758). Involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67 (By similarity). Essential for serine/threonine-protein phosphatase 2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed : 33108758).
See full target information PPP2R2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 76:3601-3620 PubMed30927017

2019

Cdc14 activation requires coordinated Cdk1-dependent phosphorylation of Net1 and PP2A-Cdc55 at anaphase onset.

Applications

Unspecified application

Species

Unspecified reactive species

Soraya Játiva,Ines Calabria,Yolanda Moyano-Rodriguez,Patricia Garcia,Ethel Queralt
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Product promise

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