Recombinant Human PQBP1 protein is a Human Full Length protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M P L P V A L Q T R L A K R G I L K H L E P E P E E E I I A E D Y D D D P V D Y E A T R L E G L P P S W Y K V F D P S C G L P Y Y W N A D T D L V S W L S P H D P N S V V T K S A K K L R S S N A D A E E K L D R S H D K S D R G H D K S D R S H E K L D R G H D K S D R G H D K S D R D R E R G Y D K V D R E R E R D R E R D R D R G Y D K A D R E E G K E R R H H R R E E L A P Y P K S K K A V S R K D E E L D P M D P S S Y S D A P R G T W S T G L P K R N E A K T G A D T T A A G P L F Q Q R P Y P S P G A V L R A N A E A S R T K Q Q D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Intrinsically disordered protein that acts as a scaffold, and which is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development (PubMed:10198427, PubMed:10332029, PubMed:12062018, PubMed:20410308, PubMed:23512658). Interacts with splicing-related factors via the intrinsically disordered region and regulates alternative splicing of target pre-mRNA species (PubMed:10332029, PubMed:12062018, PubMed:23512658, PubMed:20410308). May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery (PubMed:10198427). May be involved in ATXN1 mutant-induced cell death (PubMed:12062018). The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit (PubMed:12062018). Involved in the assembly of cytoplasmic stress granule, possibly by participating in the transport of neuronal RNA granules (PubMed:21933836). Also acts as an innate immune sensor of infection by retroviruses, such as HIV, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:26046437). Directly binds retroviral reverse-transcribed DNA in the cytosol and interacts with CGAS, leading to activate the cGAS-STING signaling pathway, triggering type-I interferon production (PubMed:26046437).
Alternative names
Polyglutamine-binding protein 1, PQBP-1, 38 kDa nuclear protein containing a WW domain, Polyglutamine tract-binding protein 1, Npw38, JM26, NPW38, PQBP1
Recommended products
Recombinant Human PQBP1 protein is a Human Full Length protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Alternative names
Polyglutamine-binding protein 1, PQBP-1, 38 kDa nuclear protein containing a WW domain, Polyglutamine tract-binding protein 1, Npw38, JM26, NPW38, PQBP1
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- O60828-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M P L P V A L Q T R L A K R G I L K H L E P E P E E E I I A E D Y D D D P V D Y E A T R L E G L P P S W Y K V F D P S C G L P Y Y W N A D T D L V S W L S P H D P N S V V T K S A K K L R S S N A D A E E K L D R S H D K S D R G H D K S D R S H E K L D R G H D K S D R G H D K S D R D R E R G Y D K V D R E R E R D R E R D R D R G Y D K A D R E E G K E R R H H R R E E L A P Y P K S K K A V S R K D E E L D P M D P S S Y S D A P R G T W S T G L P K R N E A K T G A D T T A A G P L F Q Q R P Y P S P G A V L R A N A E A S R T K Q Q D
- Accession
- Protein length
Full Length
- Predicted molecular weight
33 kDa
- Amino acids
1 to 265
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
purified by using conventional chromatography techniques.
General info
- Function
Intrinsically disordered protein that acts as a scaffold, and which is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development (PubMed:10198427, PubMed:10332029, PubMed:12062018, PubMed:20410308, PubMed:23512658). Interacts with splicing-related factors via the intrinsically disordered region and regulates alternative splicing of target pre-mRNA species (PubMed:10332029, PubMed:12062018, PubMed:23512658, PubMed:20410308). May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery (PubMed:10198427). May be involved in ATXN1 mutant-induced cell death (PubMed:12062018). The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit (PubMed:12062018). Involved in the assembly of cytoplasmic stress granule, possibly by participating in the transport of neuronal RNA granules (PubMed:21933836). Also acts as an innate immune sensor of infection by retroviruses, such as HIV, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:26046437). Directly binds retroviral reverse-transcribed DNA in the cytosol and interacts with CGAS, leading to activate the cGAS-STING signaling pathway, triggering type-I interferon production (PubMed:26046437).
- Subcellular localisation
Nucleus, Nucleus speckle
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PQBP1 also known as Polyglutamine-Binding Protein 1 is a protein that plays an important role in cellular functions. Its molecular mass is approximately 34 kDa. PQBP1 is expressed widely in human tissues with higher levels detected in the brain and muscle. It contains a WW domain which facilitates its interaction with other proteins through proline-rich sequences impacting various cellular processes such as transcriptional regulation and RNA splicing.
- Biological function summary
PQBP1 interacts with other proteins through its ability to bind to polyglutamine tracts. This protein often participates as a subunit in large complexes influencing the transcriptional machinery. It plays a role by regulating gene expression and maintaining proper RNA splicing which are critical for normal cell function and development. The ability to form complexes enables PQBP1 to impact multiple pathways critical for cellular processes highlighting its functional versatility.
- Pathways
PQBP1 actively participates in the RNA splicing pathway and transcription regulation. It interacts closely with the WNT signaling pathway which is vital for cellular proliferation and differentiation. Within these pathways PQBP1 associates with proteins like ATXN1 and SMN facilitating its role in gene expression modulation and maintaining cellular homeostasis.
- Associated diseases and disorders
PQBP1 mutations are linked to neurological conditions such as Renpenning syndrome and intellectual disability. These mutations can alter the PQBP1's interaction with other proteins including those in the WNT pathway potentially leading to disrupted cellular functions. The protein ATXN1 connected through disease pathways interacts with PQBP1 amplifying the effects of its mutations and contributing to the pathology of neurodegenerative diseases.
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1 product image
SDS-PAGE - Recombinant Human PQBP1 protein (ab126687)
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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