Recombinant Human PRAME protein is a Human Full Length protein, in the 1 to 509 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E R R R L R G S I Q S R Y I S M S V W T S P R R L V E L A G Q S L L K D E A L A I A A L E L L P R E L F P P L F M A A F D G R H S Q T L K A M V Q A W P F T C L P L G V L M K G Q H L H L E T F K A V L D G L D V L L A Q E V R P R R W K L Q V L D L R K N S H Q D F W T V W S G N R A S L Y S F P E P E A A Q P M T K K R K V D G L S T E A E Q P F I P V E V L V D L F L K E G A C D E L F S Y L I E K V K R K K N V L R L C C K K L K I F A M P M Q D I K M I L K M V Q L D S I E D L E V T C T W K L P T L A K F S P Y L G Q M I N L R R L L L S H I H A S S Y I S P E K E E Q Y I A Q F T S Q F L S L Q C L Q A L Y V D S L F F L R G R L D Q L L R H V M N P L E T L S I T N C R L S E G D V M H L S Q S P S V S Q L S V L S L S G V M L T D V S P E P L Q A L L E R A S A T L Q D L V F D E C G I T D D Q L L A L L P S L S H C S Q L T T L S F Y G N S I S I S A L Q S L L Q H L I G L S N L T H V L Y P V P L E S Y E D I H G T L H L E R L A Y L H A R L R E L L C E L G R P S M V W L S A N P C P H C G D R T F Y D P E P I L C P C F M P N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The protein expressed by gene PRAME functions as a transcriptional repressor, inhibiting retinoic acid signaling through retinoic acid receptors RARA, RARB, and RARG. It prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPE, OIP4, PRAME, Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, OIP-4
Recommended products
Recombinant Human PRAME protein is a Human Full Length protein, in the 1 to 509 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P78395-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E R R R L R G S I Q S R Y I S M S V W T S P R R L V E L A G Q S L L K D E A L A I A A L E L L P R E L F P P L F M A A F D G R H S Q T L K A M V Q A W P F T C L P L G V L M K G Q H L H L E T F K A V L D G L D V L L A Q E V R P R R W K L Q V L D L R K N S H Q D F W T V W S G N R A S L Y S F P E P E A A Q P M T K K R K V D G L S T E A E Q P F I P V E V L V D L F L K E G A C D E L F S Y L I E K V K R K K N V L R L C C K K L K I F A M P M Q D I K M I L K M V Q L D S I E D L E V T C T W K L P T L A K F S P Y L G Q M I N L R R L L L S H I H A S S Y I S P E K E E Q Y I A Q F T S Q F L S L Q C L Q A L Y V D S L F F L R G R L D Q L L R H V M N P L E T L S I T N C R L S E G D V M H L S Q S P S V S Q L S V L S L S G V M L T D V S P E P L Q A L L E R A S A T L Q D L V F D E C G I T D D Q L L A L L P S L S H C S Q L T T L S F Y G N S I S I S A L Q S L L Q H L I G L S N L T H V L Y P V P L E S Y E D I H G T L H L E R L A Y L H A R L R E L L C E L G R P S M V W L S A N P C P H C G D R T F Y D P E P I L C P C F M P N
- Accession
- P78395
- Protein length
- Full Length
- Predicted molecular weight
- 81.73 kDa
- Amino acids
- 1 to 509
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
The protein expressed by gene PRAME functions as a transcriptional repressor, inhibiting retinoic acid signaling through retinoic acid receptors RARA, RARB, and RARG. It prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the PRAME family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRAME also known as Preferentially Expressed Antigen in Melanoma is a protein typically weighing around 50 kDa. It is found mostly in testis tissue but also appears in several types of tumors including melanoma and some leukemias. PRAME does not show significant expression in most normal tissues making it a potential target for cancer therapies. Immunohistochemistry (IHC) techniques such as PRAME IHC and PRAME staining often use monoclonal antibodies like mAb EPR20330 to detect PRAME protein presence in various samples.
- Biological function summary
PRAME acts as a repressor of retinoic acid signaling a process important for cell differentiation and growth. It does not directly form part of a complex but interacts with components involved in retinoic acid pathways. By binding to retinoic acid receptor complexes PRAME prevents activation of genes involved in cell cycle arrest and apoptosis contributing to unchecked cellular proliferation seen in certain cancers.
- Pathways
PRAME participates in the retinoic acid and various oncogenic signaling processes. It influences the retinoic acid pathway by interfering with the retinoic acid receptor (RAR) signaling. This interference with key proteins like RAR disrupts the normal regulatory processes that typically inhibit cancer progression. PRAME's modulation of these pathways highlights its role in promoting tumor growth and survival.
- Associated diseases and disorders
PRAME has significant relevance to cancer particularly melanoma. It is often used as a biomarker for melanoma identification due to its overexpression in such tumors. Additionally PRAME's connection to acute myeloid leukemia (AML) emerges from its ability to act similarly in diverse malignant cells. In these diseases the overexpression of PRAME interferes with normal differentiation processes and allows for sustained proliferation of malignant cells suggesting its potential role as a therapeutic target or diagnostic marker in oncology.
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1 product image
SDS-PAGE - Recombinant Human PRAME protein (ab132315)
12.5% SDS-PAGE analysis of ab132315 stained with Coomassie Blue.
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