Recombinant Human PRCP protein is a Human Full Length protein, in the 1 to 496 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M G R R A L L L L L L S F L A P W A T I A L R P A L R A L G S L H L P T N P T S L P A V A K N Y S V L Y F Q Q K V D H F G F N T V K T F N Q R Y L V A D K Y W K K N G G S I L F Y T G N E G D I I W F C N N T G F M W D V A E E L K A M L V F A E H R Y Y G E S L P F G D N S F K D S R H L N F L T S E Q A L A D F A E L I K H L K R T I P G A E N Q P V I A I G G S Y G G M L A A W F R M K Y P H M V V G A L A A S A P I W Q F E D L V P C G V F M K I V T T D F R K S G P H C S E S I H R S W D A I N R L S N T G S G L Q W L T G A L H L C S P L T S Q D I Q H L K D W I S E T W V N L A M V D Y P Y A S N F L Q P L P A W P I K V V C Q Y L K N P N V S D S L L L Q N I F Q A L N V Y Y N Y S G Q V K C L N I S E T A T S S P G T L G W S Y Q A C T E V V M P F C T N G V D D M F E P H S W N L K E L S D D C F Q Q W G V R P R P S W I T T M Y G G K N I S S H T N I V F S N G E L D P W S G G G V T K D I T D T L V A V T I S E G A H H L D L R T K N A L D P M S V L L A R S L E V R H M K N W I R D F Y D S A G K Q H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
Alternative names
PCP, PRCP, Lysosomal Pro-X carboxypeptidase, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase, Prolylcarboxypeptidase
Recommended products
Recombinant Human PRCP protein is a Human Full Length protein, in the 1 to 496 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P42785-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M G R R A L L L L L L S F L A P W A T I A L R P A L R A L G S L H L P T N P T S L P A V A K N Y S V L Y F Q Q K V D H F G F N T V K T F N Q R Y L V A D K Y W K K N G G S I L F Y T G N E G D I I W F C N N T G F M W D V A E E L K A M L V F A E H R Y Y G E S L P F G D N S F K D S R H L N F L T S E Q A L A D F A E L I K H L K R T I P G A E N Q P V I A I G G S Y G G M L A A W F R M K Y P H M V V G A L A A S A P I W Q F E D L V P C G V F M K I V T T D F R K S G P H C S E S I H R S W D A I N R L S N T G S G L Q W L T G A L H L C S P L T S Q D I Q H L K D W I S E T W V N L A M V D Y P Y A S N F L Q P L P A W P I K V V C Q Y L K N P N V S D S L L L Q N I F Q A L N V Y Y N Y S G Q V K C L N I S E T A T S S P G T L G W S Y Q A C T E V V M P F C T N G V D D M F E P H S W N L K E L S D D C F Q Q W G V R P R P S W I T T M Y G G K N I S S H T N I V F S N G E L D P W S G G G V T K D I T D T L V A V T I S E G A H H L D L R T K N A L D P M S V L L A R S L E V R H M K N W I R D F Y D S A G K Q H
- Accession
- P42785
- Protein length
- Full Length
- Amino acids
- 1 to 496
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
- Sequence similarities
Belongs to the peptidase S28 family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PRCP protein also known as Prolylcarboxypeptidase is an enzyme with a molecular weight of around 55 kDa. It functions mechanically by cleaving C-terminal proline residues from peptides. This protein localizes in different tissues including the kidney brain and liver. Its expression is not limited to these areas and can be found in varying levels throughout other tissues in the body.
- Biological function summary
PRCP plays a role in several physiological processes including blood pressure regulation and appetite control. It acts as part of a complex involved in degrading peptide hormones like angiotensin II. By modulating these peptide hormones PRCP influences the renin-angiotensin system which is critical for blood pressure homeostasis. The regulation of neuropeptides also impacts the brain's control of appetite and metabolism.
- Pathways
PRCP integrates into the renin-angiotensin-aldosterone system (RAAS) and the kallikrein-kinin system. In the RAAS PRCP influences angiotensin II levels which affects vasoconstriction and blood pressure. It has interactions with proteins such as angiotensin-converting enzyme (ACE) within these pathways enhancing its role in vascular biology and fluid balance.
- Associated diseases and disorders
An abnormal activity of PRCP links to hypertension and obesity. Hypertension connects to its activity on the RAAS pathway where it modulates angiotensin II influencing blood vessel constriction. In obesity altered PRCP activity affects neuropeptides in the brain impacting appetite and energy balance. PRCP's modulation of these systems makes it a potential target for therapeutic interventions in these conditions.
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1 product image
SDS-PAGE - Recombinant Human PRCP protein (ab159182)
ab159182 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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