Recombinant Human PRCP protein (His tag) is a Human Full Length protein, in the 31 to 496 aa range, expressed in HEK 293, with =50% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- =50% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S L H L P T N P T S L P A V A K N Y S V L Y F Q Q K V D H F G F N T V K T F N Q R Y L V A D K Y W K K N G G S I L F Y T G N E G D I I W F C N N T G F M W D V A E E L K A M L V F A E H R Y Y G E S L P F G D N S F K D S R H L N F L T S E Q A L A D F A E L I K H L K R T I P G A E N Q P V I A I G G S Y G G M L A A W F R M K Y P H M V V G A L A A S A P I W Q F E D L V P C G V F M K I V T T D F R K S G P H C S E S I H R S W D A I N R L S N T G S G L Q W L T G A L H L C S P L T S Q D I Q H L K D W I S E T W V N L A M V D Y P Y A S N F L Q P L P A W P I K V V C Q Y L K N P N V S D S L L L Q N I F Q A L N V Y Y N Y S G Q V K C L N I S E T A T S S L G T L G W S Y Q A C T E V V M P F C T N G V D D M F E P H S W N L K E L S D D C F Q Q W G V R P R P S W I T T M Y G G K N I S S H T N I V F S N G E L D P W S G G G V T K D I T D T L V A V T I S E G A H H L D L R T K N A L D P M S V L L A R S L E V R H M K N W I R D F Y D S A G K Q H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
Alternative names
PCP, PRCP, Lysosomal Pro-X carboxypeptidase, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase, Prolylcarboxypeptidase
Recommended products
Recombinant Human PRCP protein (His tag) is a Human Full Length protein, in the 31 to 496 aa range, expressed in HEK 293, with =50% purity and suitable for SDS-PAGE.
Key facts
- Purity
- =50% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P42785-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- S L H L P T N P T S L P A V A K N Y S V L Y F Q Q K V D H F G F N T V K T F N Q R Y L V A D K Y W K K N G G S I L F Y T G N E G D I I W F C N N T G F M W D V A E E L K A M L V F A E H R Y Y G E S L P F G D N S F K D S R H L N F L T S E Q A L A D F A E L I K H L K R T I P G A E N Q P V I A I G G S Y G G M L A A W F R M K Y P H M V V G A L A A S A P I W Q F E D L V P C G V F M K I V T T D F R K S G P H C S E S I H R S W D A I N R L S N T G S G L Q W L T G A L H L C S P L T S Q D I Q H L K D W I S E T W V N L A M V D Y P Y A S N F L Q P L P A W P I K V V C Q Y L K N P N V S D S L L L Q N I F Q A L N V Y Y N Y S G Q V K C L N I S E T A T S S L G T L G W S Y Q A C T E V V M P F C T N G V D D M F E P H S W N L K E L S D D C F Q Q W G V R P R P S W I T T M Y G G K N I S S H T N I V F S N G E L D P W S G G G V T K D I T D T L V A V T I S E G A H H L D L R T K N A L D P M S V L L A R S L E V R H M K N W I R D F Y D S A G K Q H
- Accession
- P42785
- Protein length
- Full Length
- Predicted molecular weight
- 53 kDa
- Amino acids
- 31 to 496
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
- Sequence similarities
Belongs to the peptidase S28 family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PRCP protein also known as Prolylcarboxypeptidase is an enzyme with a molecular weight of around 55 kDa. It functions mechanically by cleaving C-terminal proline residues from peptides. This protein localizes in different tissues including the kidney brain and liver. Its expression is not limited to these areas and can be found in varying levels throughout other tissues in the body.
- Biological function summary
PRCP plays a role in several physiological processes including blood pressure regulation and appetite control. It acts as part of a complex involved in degrading peptide hormones like angiotensin II. By modulating these peptide hormones PRCP influences the renin-angiotensin system which is critical for blood pressure homeostasis. The regulation of neuropeptides also impacts the brain's control of appetite and metabolism.
- Pathways
PRCP integrates into the renin-angiotensin-aldosterone system (RAAS) and the kallikrein-kinin system. In the RAAS PRCP influences angiotensin II levels which affects vasoconstriction and blood pressure. It has interactions with proteins such as angiotensin-converting enzyme (ACE) within these pathways enhancing its role in vascular biology and fluid balance.
- Associated diseases and disorders
An abnormal activity of PRCP links to hypertension and obesity. Hypertension connects to its activity on the RAAS pathway where it modulates angiotensin II influencing blood vessel constriction. In obesity altered PRCP activity affects neuropeptides in the brain impacting appetite and energy balance. PRCP's modulation of these systems makes it a potential target for therapeutic interventions in these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human PRCP protein (His tag) (ab198658)
10% SDS-Page analysis of ab198658 (1 μg)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com