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AB181900

Recombinant Human PREP1 protein

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Recombinant Human PREP1 protein is a Human Full Length protein, in the 1 to 436 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PREP1, PKNOX1, Homeobox protein PKNOX1, Homeobox protein PREP-1, PBX/knotted homeobox 1

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SDS-PAGE - Recombinant Human PREP1 protein (AB181900)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PREP1 protein (AB181900)

15% SDS-PAGE analysis of 3ug ab181900 stained with Coomassie Blue.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P55347

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PKNOX1.1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYRHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLLSGEPGSPYSPVQSQQIQSAITGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRFWPDSIASGVAQPPPSELTMSEGAVVTITTPVNMNVDSLQSLSSDGATLAVQQVMMAGQSEDESVDSTEEDAGALAPAHISGLVLENSDSLQ","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":436,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P55347","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PREP1 also called PBX/knotted 1 homeobox 1 (PKNOX1) is a transcription factor. It weighs approximately 45 kDa. Expression of PREP1 is mainly found in the nucleus of various cell types particularly in tissues with rapid cell proliferation like embryonic development regions spleen and thymus. PREP1 is part of the homeobox gene family and is involved in regulating genes necessary for cellular differentiation and growth.
Biological function summary

The function of PREP1 involves the regulation of numerous developmental processes. It interacts with proteins to form heterodimeric complexes like the PREP1-PBX complex which modulate the transcription of specific target genes. This regulation is important for proper development during embryogenesis and also contributes to hematopoietic cell differentiation. Through these mechanisms PREP1 influences processes underlying organogenesis and cellular identity.

Pathways

The function of PREP1 integrates into the broader context of key regulatory cascades including the Hox gene network and the insulin-signaling pathway. PREP1 interacts with PBX proteins within these pathways affecting the transcriptional programs they control. In particular by influencing the Hox pathway PREP1 plays a role in the spatial patterning of body structures and anterior-posterior axis formation in the embryo. This regulatory activity highlights its importance in the intricate orchestration of developmental cues.

Mutations or dysregulation of PREP1 impact conditions like cancer and diabetes. Abnormal PREP1 expression has been connected to tumorigenesis in organs where its developmental control is critical. For example studies show altered PREP1 levels in certain leukemias with implications for progression and outcome. In diabetes the interplay between PREP1 and insulin-signaling pathways can affect glucose metabolism and pancreatic function linking PREP1 to metabolic disorders where it may cooperate with proteins like FOXO1 in influencing glucose homeostasis.
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Specifications

Form

Liquid

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General info

Function

Activates transcription in the presence of PBX1A and HOXA1.

Sequence similarities

Belongs to the TALE/MEIS homeobox family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Activates transcription in the presence of PBX1A and HOXA1.
See full target information PKNOX1
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AB154587

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Product promise

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