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AB125604

Recombinant human PRKX protein

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Recombinant human PRKX protein is a Human Full Length protein, in the 1 to 358 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PKX1, PRKX, cAMP-dependent protein kinase catalytic subunit PRKX, PrKX, Protein kinase X, Protein kinase X-linked, Serine/threonine-protein kinase PRKX, Protein kinase PKX1

2 Images
Functional Studies - Recombinant human PRKX protein (AB125604)
  • FuncS

Unknown

Functional Studies - Recombinant human PRKX protein (AB125604)
SDS-PAGE - Recombinant human PRKX protein (AB125604)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PRKX protein (AB125604)

SDS Page analysis of ab125604

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity was determined to be 150 nmol/min/mg

Accession

P51817

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

Primary Antibodies

AB247844

Anti-PRKX antibody [EPR4840] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-PRKX antibody [EPR4840] - BSA and Azide free (AB247844)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204872 (PAK peptide substrate) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"73 kDa","actualMolecularWeight":null,"aminoAcidEnd":358,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P51817","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKX also known as Protein Kinase X is a serine/threonine-protein kinase with a molecular mass of about 37 kDa. This protein is part of the cAMP-dependent protein kinase family. Scientists observe PRKX expression mainly in the kidney heart and central nervous system. It helps regulate multiple functions by phosphorylating various substrates which are important to cell signaling and communication. The presence of the protein in specific tissues suggests a significant role in development and organ function.
Biological function summary

The protein functions as an important regulator in cellular signaling processes. PRKX interacts with other proteins as a monomer rather than as part of a larger complex. Its activity influences cellular growth differentiation and gene expression. The kinase activity shows high involvement in developmental pathways across numerous cell types. Additionally its interactions affect cellular features like polarization and motility which are important for normal physiological processes.

Pathways

PRKX serves important roles in the PKA signaling pathway and the renal development pathway. PKA signaling involves cAMP as a second messenger and PRKX acts downstream as a phosphorylating agent for various targets within the cell. In the renal development pathway PRKX collaborates with other protein kinases like PRKY which shares a similar sequence and function reflecting redundancy and specialization in developmental roles in kidneys.

The role of PRKX becomes significant in congenital kidney disorders particularly certain types of cystic kidney diseases. The protein's influence on renal development highlights its connection to these conditions. Additionally anomalies in PRKX expression or function can link to central nervous system disorders influencing neurological development and function. Within these contexts PRKX often interacts with related protein networks involving kinases and substrates important for maintaining cellular health and functionality.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Post-translational modifications

Phosphorylated; autophosphorylates in vitro.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
See full target information PRKX
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Product promise

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