Recombinant human PRMT1 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
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- Nucleic acids research 50:11799-118192022Type I arginine methyltransferases are intervention points to unveil the oncogenic Epstein-Barr virus to the immune system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaelle Angrand,Alicia Quillévéré,Nadège Loaëc,Van-Trang Dinh,Ronan Le Sénéchal,Rahima Chennoufi,Patricia Duchambon,Marc Keruzoré,Rodrigo Prado Martins,Marie-Paule Teulade-Fichou,Robin Fåhraeus,Marc BlondelPubMed 36350639
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus MBP tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M H H H H H H M K I E E G K L V I W I N G D K G Y N G L A E V G K K F E K D T G I K V T V E H P D K L E E K F P Q V A A T G D G P D I I F W A H D R F G G Y A Q S G L L A E I T P D K A F Q D K L Y P F T W D A V R Y N G K L I A Y P I A V E A L S L I Y N K D L L P N P P K T W E E I P A L D K E L K A K G K S A L M F N L Q E P Y F T W P L I A A D G G Y A F K Y E N G K Y D I K D V G V D N A G A K A G L T F L V D L I K N K H M N A D T D Y S I A E A A F N K G E T A M T I N G P W A W S N I D T S K V N Y G V T V L P T F K G Q P S K P F V G V L S A G I N A A S P N K E L A K E F L E N Y L L T D E G L E A V N K D K P L G A V A L K S Y E E E L A K D P R I A A T M E N A Q K G E I M P N I P Q M S A F W Y A V R T A V I N A A S G R Q T V D E A L K D A Q T N S S S N N N N N N N N N N L G I E G R G S H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2 (PubMed:10749851, PubMed:15741314, PubMed:16879614, PubMed:18951090, PubMed:22095282, PubMed:25284789, PubMed:26575292, PubMed:26876602, PubMed:27642082, PubMed:30765518, PubMed:31257072, PubMed:38006878). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates MRE11 and TP53BP1, promoting the DNA damage response (PubMed:15741314, PubMed:16294045, PubMed:29651020). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (PubMed:18951090). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed:22095282). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789). Plays a role in regulating alternative splicing in the heart (By similarity). Methylates NPRL2 at 'Arg-78' leading to inhibition of its GTPase activator activity and then the GATOR1 complex and consequently inducing timely mTORC1 activation under methionine-sufficient conditions (PubMed:38006878).
Alternative names
HMT2, HRMT1L2, IR1B4, PRMT1, Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4
Recommended products
Recombinant human PRMT1 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus MBP tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 10,000unit/ml. Unit Definition : One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37C.
- Accession
- Q99873-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.484% Tris, 0.038% Magnesium chloride, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M H H H H H H M K I E E G K L V I W I N G D K G Y N G L A E V G K K F E K D T G I K V T V E H P D K L E E K F P Q V A A T G D G P D I I F W A H D R F G G Y A Q S G L L A E I T P D K A F Q D K L Y P F T W D A V R Y N G K L I A Y P I A V E A L S L I Y N K D L L P N P P K T W E E I P A L D K E L K A K G K S A L M F N L Q E P Y F T W P L I A A D G G Y A F K Y E N G K Y D I K D V G V D N A G A K A G L T F L V D L I K N K H M N A D T D Y S I A E A A F N K G E T A M T I N G P W A W S N I D T S K V N Y G V T V L P T F K G Q P S K P F V G V L S A G I N A A S P N K E L A K E F L E N Y L L T D E G L E A V N K D K P L G A V A L K S Y E E E L A K D P R I A A T M E N A Q K G E I M P N I P Q M S A F W Y A V R T A V I N A A S G R Q T V D E A L K D A Q T N S S S N N N N N N N N N N L G I E G R G S H
- Accession
- Q99873
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus, MBP tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab89007 is purified using conventional chromatography techniques.
General info
- Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2 (PubMed:10749851, PubMed:15741314, PubMed:16879614, PubMed:18951090, PubMed:22095282, PubMed:25284789, PubMed:26575292, PubMed:26876602, PubMed:27642082, PubMed:30765518, PubMed:31257072, PubMed:38006878). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates MRE11 and TP53BP1, promoting the DNA damage response (PubMed:15741314, PubMed:16294045, PubMed:29651020). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (PubMed:18951090). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed:22095282). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789). Plays a role in regulating alternative splicing in the heart (By similarity). Methylates NPRL2 at 'Arg-78' leading to inhibition of its GTPase activator activity and then the GATOR1 complex and consequently inducing timely mTORC1 activation under methionine-sufficient conditions (PubMed:38006878).
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
- Post-translational modifications
Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation (By similarity). Ubiquitination is regulated by acetylation at Lys-228 and Lys-233 (By similarity). Polyubiquitinated by E3 ubiquitin-protein ligase TRIM48, leading to suppression of MAP3K5/ASK1 methylation and subsequent MAP3K5 activation (PubMed:29186683).
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PRMT1 also known as protein arginine methyltransferase 1 is an important enzyme for post-translational modification. With a molecular mass of approximately 41 kDa PRMT1 is involved in the methylation of arginine residues on target proteins. This enzyme is ubiquitously expressed across various tissues indicating its widespread importance in cellular functions. PRMT1 functions in both the nucleus and cytoplasm impacting diverse cellular processes through its enzymatic activity.
- Biological function summary
PRMT1 participates in several essential cellular functions including signal transduction gene expression and RNA processing. It methylates histones and non-histone proteins influencing chromatin structure and function. PRMT1 does not normally function as part of a complex but it interacts individually with different substrates to exert its effects. PRMT1 activity is essential in regulating transcriptional activation and repression highlighting its significant role in cellular regulation.
- Pathways
PRMT1 plays a central role in the regulation of the glucocorticoid receptor signaling and p53 pathways. In glucocorticoid receptor signaling PRMT1 modifies transcription factors affecting their ability to bind DNA and regulate gene expression. In the p53 pathway PRMT1 methylates p53 itself impacting cell cycle arrest and apoptotic functions. These pathways demonstrate PRMT1’s interaction with other proteins like the p53 protein highlighting its integrative role in essential cellular processes.
- Associated diseases and disorders
PRMT1 is implicated in cancer and cardiovascular diseases. Aberrant expression or activity of PRMT1 associates with tumor progression and poor prognosis in cancers making it a target for therapeutic intervention. Additionally PRMT1 contributes to the pathogenesis of cardiovascular diseases by influencing the expression of genes linked to cardiac hypertrophy. PRMT1 interacts with the p53 protein impacting cancer development while also being connected through its regulatory function on p53-associated genes in these diseases.
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SDS-PAGE - Recombinant human PRMT1 protein (ab89007)
15% SDS-PAGE analysis of 3μg ab89007
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