Recombinant human PRMT1 protein (Active)
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Recombinant human PRMT1 protein (Active) is a Human Full Length protein, in the 2 to 371 aa range, expressed in Baculovirus infected Sf9 cells, with >68%, suitable for SDS-PAGE, FuncS.
View Alternative Names
HMT2, HRMT1L2, IR1B4, PRMT1, Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4
- FuncS
Supplier Data
Functional Studies - Recombinant human PRMT1 protein (Active) (AB198122)
Activity assay using ab198122.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human PRMT1 protein (Active) (AB198122)
10% SDS-PAGE analysis of 1.5 μg ab198122 with Coomassie staining.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRMT1 participates in several essential cellular functions including signal transduction gene expression and RNA processing. It methylates histones and non-histone proteins influencing chromatin structure and function. PRMT1 does not normally function as part of a complex but it interacts individually with different substrates to exert its effects. PRMT1 activity is essential in regulating transcriptional activation and repression highlighting its significant role in cellular regulation.
Pathways
PRMT1 plays a central role in the regulation of the glucocorticoid receptor signaling and p53 pathways. In glucocorticoid receptor signaling PRMT1 modifies transcription factors affecting their ability to bind DNA and regulate gene expression. In the p53 pathway PRMT1 methylates p53 itself impacting cell cycle arrest and apoptotic functions. These pathways demonstrate PRMT1’s interaction with other proteins like the p53 protein highlighting its integrative role in essential cellular processes.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2 (PubMed : 10749851, PubMed : 15741314, PubMed : 16879614, PubMed : 18951090, PubMed : 22095282, PubMed : 25284789, PubMed : 26575292, PubMed : 26876602, PubMed : 27642082, PubMed : 30765518, PubMed : 31257072, PubMed : 38006878). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed : 26575292). Methylates MRE11 and TP53BP1, promoting the DNA damage response (PubMed : 15741314, PubMed : 16294045, PubMed : 29651020). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (PubMed : 18951090). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed : 25284789). Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed : 22095282). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed : 25284789). Plays a role in regulating alternative splicing in the heart (By similarity). Methylates NPRL2 at 'Arg-78' leading to inhibition of its GTPase activator activity and then the GATOR1 complex and consequently inducing timely mTORC1 activation under methionine-sufficient conditions (PubMed : 38006878).
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
Post-translational modifications
Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation (By similarity). Ubiquitination is regulated by acetylation at Lys-228 and Lys-233 (By similarity). Polyubiquitinated by E3 ubiquitin-protein ligase TRIM48, leading to suppression of MAP3K5/ASK1 methylation and subsequent MAP3K5 activation (PubMed:29186683).. Acetylation at Lys-228 and Lys-233 regulates ubiquitination by the SCF(FBXL17) complex. Acetylated at Lys-233 by p300/EP300. Deacetylated at Lys-228 and Lys-233 by SIRT1.
Subcellular localisation
Nucleus
Target data
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Product promise
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