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AB271723

Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus)

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Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus) is a Human Full Length protein, in the 2 to 845 aa range, expressed in Baculovirus infected Sf9 cells, with >83%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PRMT10, PRMT9, Protein arginine N-methyltransferase 9, Protein arginine N-methyltransferase 10

2 Images
Functional Studies - Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus) (AB271723)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus) (AB271723)

Specific activity of ab271723.

SDS-PAGE - Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus) (AB271723)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PRMT10 protein (Active) (DDDDK N-Terminus + Strep C-Terminus) (AB271723)

SDS-PAGE analysis of 2 μg ab271723.

Key facts

Purity

>83% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus Strep tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Assay Description: 10 μl reaction mix containing assay buffer with S-adenosylmethionine, specific PRMT9 substrate, and PRMT9 (30-100 ng) added to the wells. Add antibody against methylated Arg residue and A-screen Acceptor beads. Incubate for 30 mins, then add GSH-conjugated Donor beads followed by Alpha Screening detection.

Accession

Q6P2P2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB122374

Anti-PRMT10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PRMT10 antibody (AB122374)

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Proteins & Peptides

AB89007

Recombinant human PRMT1 protein (His tag N-Terminus + MBP tag N-Terminus)

SDS-PAGE - Recombinant human PRMT1 protein (His tag N-Terminus + MBP tag N-Terminus) (AB89007)

  • 0
  • 0
Primary Antibodies

AB190892

Anti-PRMT1 antibody [EPR18344]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (AB190892)

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  • 0
Biochemicals

AB287098

Furamidine dihydrochloride

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SNSRPRSRRDAGGGAGAAGRDELVSRSLQSAEHCLGVQDFGTAYAHYLLVLSLAPELKHDVKETFQYTLFRWAEELDALSRIQDLLGCYEQALELFPDDEVICNSMGEHLFRMGFRDEAAGYFHKAVKLNPDFSDAKENFYRVANWLVERWHFIMLNDTKRNTIYNAAIQKAVCLGSKSVLDIGAGTGILSMFAKKAGAHSVYACELSKTMYELACDVVAANKMEAGIKLLHTKSLDIEIPKHIPERVSLVVTETVDAGLFGEGIVESLIHAWEHLLLQPKTKGESANCEKYGKVIPASAVIFGMAVECAEIRRHHRVGIKDIAGIHLPTNVKFQSPAYSSVDTEETIEPYTTEKMSRVPGGYLALTECFEIMTVDFNNLQELKSLATKKPDKIGIPVIKEGILDAIMVWFVLQLDDEHSLSTSPSEETCWEQAVYPVQDLADYWIKPGDHVMMEVSCQDCYLRIQSISVLGLECEMDVAKSFTQNKDLLSLGNEAELCSALANLQTSKPDAVEQTCILESTEIALLNNIPYHEGFKMAMSKVLSSLTPEKLYQTMDTHCQNEMSSGTGQSNTVQNILEPFYVLDVSEGFSVLPVIAGTLGQVKPYSSVEKDQHRIALDLISEANHFPKETLEFWLRHVEDESAMLQRPKSDKLWSIIILDVIEPSGLIQQEIMEKAAISRCLLQSGGKIFPQYVLMFGLLVESQTLLEENAVQGTERTLGLNIAPFINQFQVPIRVFLDLSSLPCIPLSKPVELLRLDLMTPYLNTSNREVKVYVCKSGRLTAIPFWYHMYLDEEIRLDTSSEASHWKQAAVVLDNPIQVEMGEELVLSIQHHKSNVSITVKQ","proteinLength":"Full Length","predictedMolecularWeight":"97 kDa","actualMolecularWeight":null,"aminoAcidEnd":845,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q6P2P2","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"Strep","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRMT10 also known as Protein Arginine Methyltransferase 10 participates in the methylation of arginine residues in target proteins. This enzyme functions mainly in cellular locations such as the nucleus and the cytoplasm. Weighing around 64 kDa PRMT10 modifies histones through methylation playing a critical role in transcriptional regulation and other chromatin-related processes. Expression of PRMT10 can be seen across many tissues with higher levels found in actively dividing cells due to its role in cell cycle regulation.
Biological function summary

PRMT10 affects chromatin structure and gene expression by methylating histones and other substrates. This protein often associates with complexes involved in transcriptional repression or activation. It targets histones like H3 and H4 influencing the recruitment of transcriptional machinery and chromatin remodelers. Through its enzymatic activity PRMT10 impacts processes like gene silencing DNA repair and signal transduction.

Pathways

The enzymatic activity of PRMT10 integrates with several cellular pathways. It prominently operates in the regulation of the Wnt signaling pathway and the cell cycle control pathway. PRMT10 works alongside related proteins such as β-catenin in Wnt signaling modulating gene expression patterns critical for cell growth and differentiation. Furthermore its interaction with cyclin-dependent kinases (CDKs) highlights its involvement in cell cycle checkpoints.

PRMT10 has been linked to cancer and neurodegenerative diseases. Aberrant expression and function of PRMT10 contribute to tumorigenesis by influencing oncogene and tumor suppressor gene expression. In cancer PRMT10 interacts with proteins like Myc enhancing its transcriptional activity which promotes cell proliferation. In neurodegenerative disorders altered PRMT10 activity affects neuronal gene expression highlighting its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of SF3B2. Involved in the regulation of alternative splicing of pre-mRNA (PubMed : 25737013, PubMed : 25979344).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.

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Product protocols

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Target data

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of SF3B2. Involved in the regulation of alternative splicing of pre-mRNA (PubMed : 25737013, PubMed : 25979344).
See full target information PRMT9
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