Recombinant Human PRMT2/HMT1 protein (Tagged) is a Human Full Length protein, in the 2 to 433 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A T S G D C P R S E S Q G E E P A E C S E A G L L Q E G V Q P E E F V A I A D Y A A T D E T Q L S F L R G E K I L I L R Q T T A D W W W G E R A G C C G Y I P A N H V G K H V D E Y D P E D T W Q D E E Y F G S Y G T L K L H L E M L A D Q P R T T K Y H S V I L Q N K E S L T D K V I L D V G C G T G I I S L F C A H Y A R P R A V Y A V E A S E M A Q H T G Q L V L Q N G F A D I I T V Y Q Q K V E D V V L P E K V D V L V S E W M G T C L L F E F M I E S I L Y A R D A W L K E D G V I W P T M A A L H L V P C S A D K D Y R S K V L F W D N A Y E F N L S A L K S L A V K E F F S K P K Y N H I L K P E D C L S E P C T I L Q L D M R T V Q I S D L E T L R G E L R F D I R K A G T L H G F T A W F S V H F Q S L Q E G Q P P Q V L S T G P F H P T T H W K Q T L F M M D D P V P V H T G D V V T G S V V L Q R N P V W R R H M S V A L S W A V T S R Q D P T S Q K V G E K V F P I W R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
Alternative names
HMT1, HRMT1L1, PRMT2, Protein arginine N-methyltransferase 2, Histone-arginine N-methyltransferase PRMT2
Recommended products
Recombinant Human PRMT2/HMT1 protein (Tagged) is a Human Full Length protein, in the 2 to 433 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P55345-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- A T S G D C P R S E S Q G E E P A E C S E A G L L Q E G V Q P E E F V A I A D Y A A T D E T Q L S F L R G E K I L I L R Q T T A D W W W G E R A G C C G Y I P A N H V G K H V D E Y D P E D T W Q D E E Y F G S Y G T L K L H L E M L A D Q P R T T K Y H S V I L Q N K E S L T D K V I L D V G C G T G I I S L F C A H Y A R P R A V Y A V E A S E M A Q H T G Q L V L Q N G F A D I I T V Y Q Q K V E D V V L P E K V D V L V S E W M G T C L L F E F M I E S I L Y A R D A W L K E D G V I W P T M A A L H L V P C S A D K D Y R S K V L F W D N A Y E F N L S A L K S L A V K E F F S K P K Y N H I L K P E D C L S E P C T I L Q L D M R T V Q I S D L E T L R G E L R F D I R K A G T L H G F T A W F S V H F Q S L Q E G Q P P Q V L S T G P F H P T T H W K Q T L F M M D D P V P V H T G D V V T G S V V L Q R N P V W R R H M S V A L S W A V T S R Q D P T S Q K V G E K V F P I W R
- Accession
- P55345
- Protein length
- Full Length
- Predicted molecular weight
- 50 kDa
- Amino acids
- 2 to 433
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Arginine Methyltransferase 2 (PRMT2) also known as HMT1 is a member of the protein arginine methyltransferase family. This enzyme carries out the transfer of methyl groups to arginine residues on target proteins influencing gene expression. PRMT2 has a molecular mass of about 59 kDa. It is detected widely in human tissues most notably in the liver heart and brain. This distribution indicates its involvement in a broad range of cellular activities.
- Biological function summary
PRMT2 regulates various processes through its methylation activity. It functions as a coactivator particularly in the nuclear hormone receptor pathway. This protein is part of complexes that modify chromatin affecting gene transcription regulation. Through methylation of histones and other proteins PRMT2 influences cellular differentiation and proliferation. By modifying these key components PRMT2 controls important physiological functions within cells.
- Pathways
PRMT2 interacts closely with nuclear hormone receptor signaling and pathways associated with gene regulation. These connections make it particularly important in the estrogen receptor signaling pathway where PRMT2 acts in conjunction with other proteins like PRMT1. Additionally PRMT2 has roles in influencing the PI3K/AKT pathway an important signaling pathway related to cell survival and metabolism.
- Associated diseases and disorders
PRMT2 exhibits relevant associations with conditions like cancer and neurological disorders. In cancer altered PRMT2 expression can misregulate gene expression through disrupted methylation promoting tumorigenic processes. Its dysregulation also connects to inflammation and degenerative diseases in the nervous system often involving proteins like PRMT1 as collaborators in pathology development. Understanding these links with diseases can guide therapeutic approaches involving PRMT2 modulation.
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1 product image
SDS-PAGE - Recombinant Human PRMT2/HMT1 protein (Tagged) (ab271718)
SDS-PAGE analysis of ab271718.
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