Recombinant human PRMT3 protein is a Human Full Length protein, in the 1 to 531 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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- Cancers 11:2018Protein Arginine Methyltransferase 3 Enhances Chemoresistance in Pancreatic Cancer by Methylating hnRNPA1 to Increase ABCG2 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Chuan Hsu et. al.PubMed 30577570
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M C S L A S G A T G G R G A V E N E E D L P E L S D S G D E A A W E D E D D A D L P H G K Q Q T P C L F C N R L F T S A E E T F S H C K S E H Q F N I D S M V H K H G L E F Y G Y I K L I N F I R L K N P T V E Y M N S I Y N P V P W E K E E Y L K P V L E D D L L L Q F D V E D L Y E P V S V P F S Y P N G L S E N T S V V E K L K H M E A R A L S A E A A L A R A R E D L Q K M K Q F A Q D F V M H T D V R T C S S S T S V I A D L Q E D E D G V Y F S S Y G H Y G I H E E M L K D K I R T E S Y R D F I Y Q N P H I F K D K V V L D V G C G T G I L S M F A A K A G A K K V L G V D Q S E I L Y Q A M D I I R L N K L E D T I T L I K G K I E E V H L P V E K V D V I I S E W M G Y F L L F E S M L D S V L Y A K N K Y L A K G G S V Y P D I C T I S L V A V S D V N K H A D R I A F W D D V Y G F K M S C M K K A V I P E A V V E V L D P K T L I S E P C G I K H I D C H T T S I S D L E F S S D F T L K I T R T S M C T A I A G Y F D I Y F E K N C H N R V V F S T G P Q S T K T H W K Q T V F L L E K P F S V K A G E A L K G K V T V H K S K K D P R S L T V T L T L N N S T Q T Y G L Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (PubMed:33495566).
Alternative names
HRMT1L3, PRMT3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
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Recombinant human PRMT3 protein is a Human Full Length protein, in the 1 to 531 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.
- Accession
- O60678-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M C S L A S G A T G G R G A V E N E E D L P E L S D S G D E A A W E D E D D A D L P H G K Q Q T P C L F C N R L F T S A E E T F S H C K S E H Q F N I D S M V H K H G L E F Y G Y I K L I N F I R L K N P T V E Y M N S I Y N P V P W E K E E Y L K P V L E D D L L L Q F D V E D L Y E P V S V P F S Y P N G L S E N T S V V E K L K H M E A R A L S A E A A L A R A R E D L Q K M K Q F A Q D F V M H T D V R T C S S S T S V I A D L Q E D E D G V Y F S S Y G H Y G I H E E M L K D K I R T E S Y R D F I Y Q N P H I F K D K V V L D V G C G T G I L S M F A A K A G A K K V L G V D Q S E I L Y Q A M D I I R L N K L E D T I T L I K G K I E E V H L P V E K V D V I I S E W M G Y F L L F E S M L D S V L Y A K N K Y L A K G G S V Y P D I C T I S L V A V S D V N K H A D R I A F W D D V Y G F K M S C M K K A V I P E A V V E V L D P K T L I S E P C G I K H I D C H T T S I S D L E F S S D F T L K I T R T S M C T A I A G Y F D I Y F E K N C H N R V V F S T G P Q S T K T H W K Q T V F L L E K P F S V K A G E A L K G K V T V H K S K K D P R S L T V T L T L N N S T Q T Y G L Q
- Accession
- O60678
- Protein length
- Full Length
- Predicted molecular weight
- 97 kDa
- Amino acids
- 1 to 531
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (PubMed:33495566).
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein arginine methyltransferase 3 (PRMT3) plays an important role in the methylation of arginine residues on various substrates which influences protein-protein interactions signal transduction and gene expression. Also known as HRMT1L3 PRMT3 has an approximate molecular weight of 59 kDa. Researchers find this enzyme mainly in the cytoplasm although it can also localize in the nucleus. Expression of PRMT3 happens across multiple tissues with notable presence in the liver and lungs.
- Biological function summary
PRMT3 methylates ribosomal protein S2 an activity that suggests its participation in ribosome assembly and nucleolar functions. It forms a complex with ribosomal proteins and several metabolic enzymes which highlight its involvement in protein synthesis and cellular metabolic regulation. The activity of PRMT3 regulates the biogenesis of ribosomes which are central to protein production in cells.
- Pathways
PRMT3 influences various cellular processes like the ribosome biogenesis and the mTOR signaling pathway. Its interaction with the ribosome synthesis pathway aids in the maturation and assembly of ribosomal subunits. In the mTOR pathway PRMT3 relates to S6 kinase and eukaryotic initiation factors affecting cell growth and metabolism through the regulation of protein synthesis.
- Associated diseases and disorders
PRMT3 may influence the development of metabolic syndromes and certain cancers. Studies suggest a potential link between PRMT3 dysregulation and the onset of insulin resistance making it significant in the context of type 2 diabetes. Additionally abnormal PRMT3 expression connects with progression of malignancies potentially through interacting with oncogenic proteins and altering signaling pathways that regulate cell cycle and apoptosis.
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2 product images
Functional Studies - Recombinant human PRMT3 protein (ab167953)
The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.
SDS-PAGE - Recombinant human PRMT3 protein (ab167953)
SDS-PAGE analysis of ab167953.
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