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AB196434

Recombinant human PRMT6 protein (His tag N-Terminus)

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Recombinant human PRMT6 protein (His tag N-Terminus) is a Human Full Length protein, in the 2 to 375 aa range, expressed in Escherichia coli, with >45%, suitable for SDS-PAGE, FuncS.

View Alternative Names

HRMT1L6, PRMT6, Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6

3 Images
Functional Studies - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)

Specific activity of ab196434.

SDS-PAGE - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)

SDS-PAGE analysis of 3 μg of ab196434 on a 10% SDS-PAGE gel stained with Coomassie.

SDS-PAGE - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PRMT6 protein (His tag N-Terminus) (AB196434)

SDS-PAGE analysis of 2 μg of ab196434 on a 4-20% SDS-PAGE gel stained with Coomassie.

Key facts

Purity

>45% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity 0.015 pmol/min/Âμg
Assay Conditions: 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 20 μM S-adenosylmethionine, and 1-5 ng methyltransferase PRMT6) is added to microwells coated with histone substrate. Incubate at 30°C for 2 hr. Add antibody against methylated Arg residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.

Accession

Q96LA8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.36% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SQPKKRKLESGGGGEGGEGTEEEDGAEREAALERPRRTKRERDQLYYECYSDVSVHEEMIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED","proteinLength":"Full Length","predictedMolecularWeight":"43 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96LA8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRMT6 or Protein Arginine Methyltransferase 6 is an enzyme that adds methyl groups to arginine residues on target proteins. It is a member of the PRMT family and has a molecular mass of approximately 42 kDa. This enzyme is widely expressed in nucleous where it modifies histones and various nuclear proteins influencing chromatin structure and gene expression.
Biological function summary

PRMT6 plays a pivotal role in regulating transcription by methylating histone H3 at arginine 2 (H3R2) which suppresses gene expression. It forms part of larger chromatin remodeling complexes that modulate access to DNA. PRMT6 also interacts with p53 an important regulator of the cell cycle impacting its transcriptional activity and stability.

Pathways

PRMT6 is central to epigenetic and transcriptional regulation pathways. It functions alongside proteins like p53 and Mdm2 within the DNA damage response pathway where it influences cell proliferation and repair processes. This enzyme further relates to the Akt signaling pathway which affects cell growth and survival although it is not the primary pathway.

PRMT6 is implicated in cancer and systemic lupus erythematosus (SLE). In cancer deregulation of PRMT6 can lead to uncontrolled cell growth by affecting genes related to cell cycle and proliferation often interacting with protein p53. In systemic lupus erythematosus altered PRMT6 activity may contribute to abnormal immune responses associating with proteins involved in autoimmunity.
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Specifications

Form

Liquid

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General info

Function

Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910, PubMed : 30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed : 17898714, PubMed : 18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed : 19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed : 16600869). Methylates HMGA1 (PubMed : 16157300, PubMed : 16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed : 17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed : 30420520).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.

Post-translational modifications

Automethylation enhances its stability and antiretroviral activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910, PubMed : 30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed : 17898714, PubMed : 18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed : 19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed : 16600869). Methylates HMGA1 (PubMed : 16157300, PubMed : 16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed : 17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed : 30420520).
See full target information PRMT6
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