Recombinant human PRMT6 protein (Active)
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(1 Publication)
Recombinant human PRMT6 protein (Active) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
HRMT1L6, PRMT6, Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6
- FuncS
Supplier Data
Functional Studies - Recombinant human PRMT6 protein (Active) (AB268890)
The specific activity of ab268890 was 1.4 nmol/min/mg in a methyltransferase assay using histone H3 peptide (1-21) as substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human PRMT6 protein (Active) (AB268890)
SDS-PAGE analysis of ab268890.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRMT6 plays a pivotal role in regulating transcription by methylating histone H3 at arginine 2 (H3R2) which suppresses gene expression. It forms part of larger chromatin remodeling complexes that modulate access to DNA. PRMT6 also interacts with p53 an important regulator of the cell cycle impacting its transcriptional activity and stability.
Pathways
PRMT6 is central to epigenetic and transcriptional regulation pathways. It functions alongside proteins like p53 and Mdm2 within the DNA damage response pathway where it influences cell proliferation and repair processes. This enzyme further relates to the Akt signaling pathway which affects cell growth and survival although it is not the primary pathway.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910, PubMed : 30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182, PubMed : 19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed : 17898714, PubMed : 18077460, PubMed : 18079182). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed : 17898714, PubMed : 18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed : 19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed : 16600869). Methylates HMGA1 (PubMed : 16157300, PubMed : 16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed : 17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed : 30420520).
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
Post-translational modifications
Automethylation enhances its stability and antiretroviral activity.
Subcellular localisation
Nucleus
Target data
Publications (1)
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Journal of medicinal chemistry 68:4373-4381 PubMed39961800
2025
Applications
Unspecified application
Species
Unspecified reactive species
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