JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB268892

Recombinant human PRMT8 protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PRMT8 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

HRMT1L3, HRMT1L4, PRMT8, Protein arginine N-methyltransferase 8, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4

2 Images
Functional Studies - Recombinant human PRMT8 protein (Active) (AB268892)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PRMT8 protein (Active) (AB268892)

The specific activity of ab268892 was 35 nmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.

SDS-PAGE - Recombinant human PRMT8 protein (Active) (AB268892)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PRMT8 protein (Active) (AB268892)

SDS-PAGE analysis of ab268892.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268892 was 35 nmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.

Accession

Q9NR22

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NR22","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein arginine methyltransferase 8 commonly referred to as PRMT8 catalyzes the transfer of methyl groups to arginine residues on target proteins. It is part of the PRMT family and possesses a molecular mass of approximately 42 kDa. PRMT8 shares sequence similarity with PRMT1 but shows unique tissue-specific expression. Notably it is mainly found in the brain where it localizes to the plasma membrane suggesting specialized functions in neuronal cells.
Biological function summary

PRMT8 contributes to modulating signal transduction pathways in neurons. It plays a role in the post-translational modification of proteins which affects their interaction and stability. Though not known to form any stable large complexes PRMT8's methyltransferase activity modifies substrate proteins influencing their cellular functions. In neurons this activity may regulate synaptic plasticity or neural communication through methylation of proteins involved in signal transduction.

Pathways

Arginine methylation by PRMT8 influences several key signaling pathways including the phosphoinositide and protein kinase pathways. PRMT8's activity affects proteins such as Src family kinases which are important in transmitting signals for cell growth and differentiation. Interaction with these pathways suggests that PRMT8 serves as a modulator of complex cellular signaling mechanisms balancing neuronal response and adaptation.

Abnormalities in PRMT8 activity have associations with neurodegenerative diseases and brain-related disorders including Alzheimer's disease. Dysregulation of PRMT8 may affect amyloid precursor protein processing implicating its role along with proteins such as tau in disease pathology. Understanding PRMT8's involvement in these conditions could inform therapeutic strategies that target arginine methylation to ameliorate or prevent disease progression.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer (PubMed : 16051612, PubMed : 17925405, PubMed : 26529540, PubMed : 26876602). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS (PubMed : 18320585).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT8 subfamily.

style
left-column

Product protocols

style
left-column

Target data

S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer (PubMed : 16051612, PubMed : 17925405, PubMed : 26529540, PubMed : 26876602). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS (PubMed : 18320585).
See full target information PRMT8
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB268890

Recombinant human PRMT6 protein (Active)

Functional Studies - Recombinant human PRMT6 protein (Active) (AB268890)

  • 0
  • 0
Proteins & Peptides

AB268888

Recombinant human CARM1 protein (Active)

Functional Studies - Recombinant human CARM1 protein (Active) (AB268888)

  • 0
  • 0
Proteins & Peptides

AB268975

Recombinant human KMT3C / SMYD2 protein (Active)

Functional Studies - Recombinant human KMT3C / SMYD2 protein (Active) (AB268975)

  • 0
  • 0
Proteins & Peptides

AB316439

Recombinant Human BCMA Protein Standard (His tag)

Sandwich ELISA - Recombinant Human BCMA Protein Standard (His tag) (AB316439)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com