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AB92843

Recombinant Human Procalcitonin protein

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(1 Publication)

Recombinant Human Procalcitonin protein is a Human Full Length protein, in the 26 to 141 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, WB.

View Alternative Names

CALC1, CALCA, Calcitonin

3 Images
Western blot - Recombinant Human Procalcitonin protein (AB92843)
  • WB

Unknown

Western blot - Recombinant Human Procalcitonin protein (AB92843)

ab92843 used in Western Blot. Figure : 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue.

Human Procalcitonin has a predicted MW of 12.8 kDa.

All lanes:

Western blot - Recombinant Human Procalcitonin protein (ab92843)

false

Western blot - Recombinant Human Procalcitonin protein (AB92843)
  • WB

Unknown

Western blot - Recombinant Human Procalcitonin protein (AB92843)

All lanes:

Anti-Procalcitonin antibody [13F2] (<a href='/en-us/products/unavailable/procalcitonin-antibody-13f2-ab90489'>ab90489</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Procalcitonin protein (ab92843) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

true

Exposure time: 3min

SDS-PAGE - Recombinant Human Procalcitonin protein (AB92843)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Procalcitonin protein (AB92843)

1ug of ab92843 was resolved on a 4-20% polyacrylamide, Tris-Glycine gel under non-reducing and reducing (+) conditions Recombinant Human Procalcitonin has a predicted molecular weight of 12.8kDa.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P01258

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab92843 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/procalcitonin-antibody-13f2-ab90489'>ab90489</a>.</p>" } } }
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AB221828

Human Procalcitonin ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Sequence info

[{"sequence":"APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN","proteinLength":"Full Length","predictedMolecularWeight":"12.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":141,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01258","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procalcitonin also known as PCT is a protein precursor of the hormone calcitonin. It has a molecular mass of about 13 kilodaltons. Procalcitonin is expressed in the thyroid gland typically but can also be produced in other tissues like the liver and kidney during inflammation or sepsis. It gets increasingly produced in response to bacterial infections making it a significant marker for conditions involving systemic inflammation.
Biological function summary

The function of procalcitonin is related to its precursor status for calcitonin which helps regulate calcium homeostasis. During infections PCT levels rise without its cleavage into active calcitonin indicating that its biological role extends beyond calcium regulation. It does not form part of any larger protein complex in its marker role. When released in response to bacterial infections procalcitonin serves as an indicator for the severity of the infection which is particularly useful in distinguishing bacterial from viral infections.

Pathways

Procalcitonin gets involved in the immune response pathway primarily due to its expression during bacterial infections. An important pathway is the inflammatory response where procalcitonin production gets triggered serving as a mediator in immune system activation. It is related to cytokines in the immune response which elevated PCT can represent indirectly indicating the presence and level of infection-driven inflammation.

Procalcitonin levels are used significantly in diagnosing and managing sepsis and bacterial infections. Procalcitonin concentrations rise sharply in a bacterial systemic infection and help differentiate it from viral infections an important diagnostic criterion. Additionally in sepsis another important protein involved is C-reactive protein (CRP) often used alongside PCT as a biomarker for inflammatory states in the body. This relationship underlines the importance of PCT in managing infectious diseases through targeted diagnostic pathways.
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Specifications

Form

Lyophilized

Additional notes

>95% as determined by RP-HPLC analyses. Purified using standard chromatographical techniques.

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General info

Function

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.

Sequence similarities

Belongs to the calcitonin family.

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Product protocols

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Target data

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
See full target information CALCA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Analyst 144:6225-6230 PubMed31555776

2019

The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes.

Applications

Unspecified application

Species

Unspecified reactive species

Fida'a Alshawawreh,Fabio Lisi,Nicholas Ariotti,Padmavathy Bakthavathsalam,Tania Benedetti,Richard D Tilley,J Justin Gooding
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB53369

Recombinant Human Procalcitonin protein

proteins-peptides

recombinant-human-procalcitonin-protein-ab53369

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(0 reviews)

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