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AB158154

Recombinant Human procollagen type IIA protein (GST tag N-Terminus)

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Recombinant Human procollagen type IIA protein (GST tag N-Terminus) is a Human Full Length protein, in the 1220 to 1487 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

1 Images
SDS-PAGE - Recombinant Human procollagen type IIA protein (GST tag N-Terminus) (AB158154)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human procollagen type IIA protein (GST tag N-Terminus) (AB158154)

ab158154 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P02458

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Proteins & Peptides

AB314201

Native Bovine Collagen protein (FITC)

  • 0
  • 0
Primary Antibodies

AB307674

Anti-Collagen II antibody [EPR27418-25]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody [EPR27418-25] (AB307674)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB184993

Anti-Collagen III antibody [EPR17673]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1487,"aminoAcidStart":1220,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P02458","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen type IIA also known as COL2A1 is a precursor of type II collagen important in forming fibrillar collagen networks. This protein has a molecular mass of approximately 146 kDa. Its expression occurs largely in cartilaginous tissues particularly during the early stages of chondrogenesis. Procollagen type IIA is also found in non-skeletal tissues such as the eye indicating its diverse roles during organismal development.
Biological function summary

Procollagen type IIA contributes to the structural integrity of cartilaginous matrices supporting tissue framework and providing tensile strength. Procollagen type IIA is a precursor in the collagen synthesis pathway where it undergoes a series of enzymatic modifications before converting into mature type II collagen fibrils. This protein forms part of a larger collagen synthesis complex and interacts with enzymes like procollagen N-proteinase playing an essential role in matrix formation.

Pathways

Procollagen type IIA participates in the extracellular matrix (ECM) organization pathway. This involvement is key in skeletal development and tissue repair. The protein interacts closely with other structural components like procollagen type IIB to support chondrogenic activities. Also it is part of the transforming growth factor-beta (TGF-beta) signaling pathway which regulates chondrocyte function and differentiation.

Procollagen type IIA mutations are linked to skeletal disorders such as stickler syndrome and achondrogenesis type II. These mutations disrupt normal collagen fiber assembly affecting cartilage function and leading to distinct skeletal abnormalities. In these disorders it is interconnected with proteins like matrix metalloproteinases (MMPs) which influence tissue remodeling and degradation exacerbating the disease conditions.
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Specifications

Form

Liquid

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General info

Function

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Sequence similarities

Belongs to the fibrillar collagen family.

Post-translational modifications

The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.. Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains.. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

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Product protocols

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Target data

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
See full target information COL2A1
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