Recombinant Human Profilin 1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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- BMC biology 20:1292022Conformational dynamics in the disordered region of human CPEB3 linked to memory consolidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Ramírez de Mingo,D Pantoja-Uceda,R Hervás,M Carrión-Vázquez,D V LaurentsPubMed 35658951
- The Journal of biological chemistry 295:15636-156492020Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbigail Allen et. al.PubMed 32883810
- Biochemical and biophysical research communications 531:459-4642020Profilin-1 is dysregulated in endometroid (type I) endometrial cancer promoting cell proliferation and inhibiting pro-inflammatory cytokine production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisanne George,Amy Winship,Kelli Sorby,Evdokia Dimitriadis,Ellen MenkhorstPubMed 32800551
- Scientific reports 7:86902017Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE M Menkhorst et. al.PubMed 28821715
- Electrophoresis 35:2681-52014Chip-based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Zienkiewicz,Krzysztof Zienkiewicz,Fernando Florido,Francisco Manuel Marco,Pedro José Romero,Antonio Jesus Castro,Juan de Dios AlchéPubMed 24888349
- PloS one 5:e136082010Profilin-1 is expressed in human atherosclerotic plaques and induces atherogenic effects on vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvren Caglayan et. al.PubMed 21049052
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A G W N A Y I D N L M A D G T C Q D A A I V G Y K D S P S V W A A V P G K T F V N I T P A E V G V L V G K D R S S F Y V N G L T L G G Q K C S V I R D S L L Q D G E F S M D L R T K S T G G A P T F N V T V T K T D K T L V L L M G K E G V H G G L I N K K C Y E M A S H L R R S Q Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Alternative names
Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1
Recommended products
Recombinant Human Profilin 1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P07737-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
M A G W N A Y I D N L M A D G T C Q D A A I V G Y K D S P S V W A A V P G K T F V N I T P A E V G V L V G K D R S S F Y V N G L T L G G Q K C S V I R D S L L Q D G E F S M D L R T K S T G G A P T F N V T V T K T D K T L V L L M G K E G V H G G L I N K K C Y E M A S H L R R S Q Y
- Accession
- P07737
- Protein length
Full Length
- Nature
Recombinant
Specifications
- Form
Liquid
- Additional notes
ab87760 is purified using conventional chromatography techniques.
General info
- Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
- Sequence similarities
Belongs to the profilin family.
- Post-translational modifications
Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Endotoxin level: < 1.0 EU per 1 µg of protein (determined by LAL method).
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Profilin 1 also known as Profilin-1 or Profilin is a small actin-binding protein with a molecular weight of approximately 15 kDa. It is expressed across various tissues including muscle brain and epithelial cells. Profilin 1 interacts with monomeric actin (G-actin) to regulate actin polymerization by binding to actin monomers. This protein also interacts with polyphosphoinositides and proline-rich sequences playing a mechanical role in balancing actin filament assembly and disassembly.
- Biological function summary
Profilin 1 acts as an important regulator of the cytoskeleton facilitating cellular processes like motility and division. It participates in the formation of actin filaments by delivering actin monomers to barbed ends promoting polymerization. Profilin 1 is part of the complex that controls actin dynamics and this ability to modulate actin structure allows it to influence cell shape and internal movement. The protein's interactions help support the actin cytoskeleton for intracellular trafficking and signal transduction.
- Pathways
Profilin 1 has important roles in the regulation of the actin cytoskeleton and Rho signaling pathways. These pathways are important in cell migration and adhesion. During cellular processes Profilin 1's interactions with actin and polyphosphoinositides link it to other proteins such as Rho GTPases which are associated with cytoskeletal dynamics. Profilin 1 can influence these pathways by modulating actin dynamics and interacting with proteins involved in these signaling cascades.
- Associated diseases and disorders
Disruptions in Profilin 1 function are linked to neurodegenerative diseases and cancer metastasis. For example aberrant Profilin 1 activity can contribute to amyotrophic lateral sclerosis (ALS) by affecting neuronal structure and transport. Dysregulation of its interaction with actin or polyphosphoinositides might also lead to enhanced cancer cell migration and invasion. It may interact with other proteins implicated in these diseases such as the ALS-related protein TDP-43 or cancer-related kinases making it significant in understanding these conditions.
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SDS-PAGE - Recombinant Human Profilin 1 protein (ab87760)
15% SDS-PAGE analysis of 3μg ab87760.
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