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AB174456

Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus)

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Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 34 to 260 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ProSAAS, Proprotein convertase subtilisin/kexin type 1 inhibitor, pro-SAAS, Proprotein convertase 1 inhibitor, PCSK1N

1 Images
SDS-PAGE - Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus) (AB174456)
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Supplier Data

SDS-PAGE - Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus) (AB174456)

15% SDS-PAGE analysis of ab174456 (3 μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UHG2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMARPVKEPRGLSAASPPLAETGAPRRFRRSVPRGEAAGAVQELARALAHLLEAERQERARAEAQEAEDQQARVLAQLLRVWGAPRNSDPALGLDDDPDAPAAQLARALLRARLDPAALAAQLVPAPVPAAALRPRPPVYDDGPAGPDAEEAGDETPDVDPELLRYLLGRILAGSADSEGVAAPRRLRRAADHDVGSELPPEGVLGALLRVKRLETPAPQVPARRLLPP","proteinLength":"Full Length","predictedMolecularWeight":"26.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":260,"aminoAcidStart":34,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UHG2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ProSAAS also known as PCSK1N (ProSAAS Neuropeptide) is a protein that acts as an inhibitor of prohormone convertases specifically PC1/3. It weighs approximately 28 kDa. ProSAAS expresses predominantly in neuroendocrine tissues especially in the brain and pancreatic islets. Its expression in these areas implies a significant role in neuroendocrine signaling.
Biological function summary

ProSAAS functions as a neuropeptide precursor influencing the maturation of other peptide hormones. It is not typically considered part of a larger protein complex but interacts with other proteins to modulate their activity. ProSAAS directly inhibits catalytic activity of PC1/3 affecting the processing and sorting of various peptide hormones and neuropeptides critical for proper brain and endocrine system function.

Pathways

Interactions between ProSAAS and its influence in the prohormone processing pathway are significant. It contributes to the regulation of the neuroendocrine signaling pathway. ProSAAS shares a regulatory network with other proteins like prohormone convertase 2 (PC2) emphasizing its role in the complex hormone maturation landscape.

Alteration in ProSAAS expression or function links to metabolic syndromes and neurodegenerative diseases. For metabolic syndromes changes in ProSAAS can impact insulin regulation linking it to diabetes via its interaction with proteins like insulin. In neurodegenerative diseases such as Alzheimer's ProSAAS appears to modulate amyloid-beta peptide formation influencing disease progression in a manner shared with amyloid precursor protein (APP).
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Specifications

Form

Liquid

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General info

Function

May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity (By similarity).. Big LEN. Endogenous ligand for GPR171. Neuropeptide involved in the regulation of feeding.. PEN. Endogenous ligand for GPR171. Neuropeptide involved in the regulation of feeding.

Post-translational modifications

Proteolytically cleaved in the Golgi.. O-glycosylated with a core 1 or possibly core 8 glycan.

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Product protocols

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Target data

May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity (By similarity).. Big LEN. Endogenous ligand for GPR171. Neuropeptide involved in the regulation of feeding.. PEN. Endogenous ligand for GPR171. Neuropeptide involved in the regulation of feeding.
See full target information PCSK1N
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