JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283430

Recombinant Human Prostate Specific Antigen protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Prostate Specific Antigen protein is a Human Full Length protein in the 25 to 261 aa range with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, mass spectrometry and HPLC. The predicted molecular weight of ab283430 protein is 26.15 kDa.

- Save time and ensure accurate results - use our Human Prostate Specific Antigen (PSA) protein as a control
- Available in different sizes to fit your experimental needs

View Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

3 Images
Mass Spectrometry - Recombinant Human Prostate Specific Antigen protein (AB283430)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human Prostate Specific Antigen protein (AB283430)

Mass determination by ESI-TOF.

Predicted MW is 26146.07 Da (+/- 10 Da by ESI-TOF). Observed MW is 26148.77

SDS-PAGE - Recombinant Human Prostate Specific Antigen protein (AB283430)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Prostate Specific Antigen protein (AB283430)

SDS-page analysis of ab283430

HPLC - Recombinant Human Prostate Specific Antigen protein (AB283430)
  • HPLC

Supplier Data

HPLC - Recombinant Human Prostate Specific Antigen protein (AB283430)

HPLC analysis of ab283430

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P07288

Animal free

Yes

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Ensure the validity of your result using our recombinant human Prostate Specific Antigen (PSA)/ Kallikrein-3 protein ab283430 as a positive control in SDS-PAGE, mass spectrometry and HPLC.


Check out our protein gel staining guide for SDS-PAGE here
style
left-column

Sequence info

[{"sequence":"IVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP","proteinLength":"Full Length","predictedMolecularWeight":"26.15 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P07288","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

style
left-column

Product protocols

style
left-column

Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
See full target information KLK3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB53774

Anti-Prostate Specific Antigen antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (AB53774)

  • 5
  • 2
Proteins & Peptides

AB317870

Recombinant Human CEACAM5/CEA Protein (His-tag)

Mass Spectrometry - Recombinant Human CEACAM5/CEA Protein (His-tag) (AB317870)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB283938

Recombinant Human PSMA Protein

HPLC - Recombinant Human PSMA Protein (AB283938)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com