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AB114372

Recombinant Human Prosurfactant Protein B

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Recombinant Human Prosurfactant Protein B is a Human Full Length protein, in the 1 to 381 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

SFTP3, SFTPB, Pulmonary surfactant-associated protein B, SP-B, 18 kDa pulmonary-surfactant protein, 6 kDa protein, Pulmonary surfactant-associated proteolipid SPL(Phe)

1 Images
SDS-PAGE - Recombinant Human Prosurfactant Protein B (AB114372)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Prosurfactant Protein B (AB114372)

12.5% SDS-PAGE with ab114372 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P07988

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Sequence info

[{"sequence":"MAESHLLQWLLLLLPTLCGPGTAAWTTSSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVGADDLCQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLKLLMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLCKSRQPEPEQEPGMSDPLPKPLRDPLPDPLLDKLVLPVLPGALQARPGPHTQDLSEQQFPIPLPYCWLCRALIKRIQAMIPKGALAVAVAQVCRVVPLVAGGICQCLAERYSVILLDTLLGRMLPQLVCRLVLRCSMDDSAGPRSPTGEWLPRDSECHLCMSVTTQAGNSSEQAIPQAMLQACVGSWLDREKCKQFVEQHTPQLLTLVPRGWDAHTTCQALGVCGTMSSPLQCIHSPDL","proteinLength":"Full Length","predictedMolecularWeight":"68.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":381,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P07988","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein B also known as surfactant protein B (SP-B) is essential for pulmonary function. It is a hydrophobic protein with an approximate mass of 8 kDa after processing from its precursor form which is larger. The protein is expressed predominantly in type II alveolar cells in the lungs and is found in lamellar bodies. SP-B functions mechanically by reducing surface tension in the alveoli facilitating efficient gaseous exchange and lung compliance.
Biological function summary

SP-B plays a significant role in the formation and stabilization of pulmonary surfactant film. It acts in conjunction with phospholipids to form a protein-lipid complex. This complex is critical for lowering surface tension at the air-liquid interface in the alveoli preventing lung collapse during exhalation. SP-B integrates lipid components into the surfactant matrix and enhances its spreading and surface activity.

Pathways

SP-B serves an integral part in lung surfactant metabolism and the phospholipid metabolic pathway. These pathways ensure the homeostasis and turnover of surfactant materials. Proteins such as surfactant proteins A (SP-A) and C (SP-C) are also involved in these pathways coordinating with SP-B to maintain surfactant function and lung physiology. Together these proteins help maintain the balance necessary for proper respiratory mechanics.

Deficiency or mutation in SP-B is associated with respiratory distress syndrome (RDS) and interstitial lung diseases. These conditions manifest due to compromised surfactant function leading to alveolar collapse. SP-B mutations can disrupt the normal surfactant pathway leading to insufficient lung function. Disorders like these often involve SP-C as well with both proteins affected in cases of congenital surfactant deficiency further impacting pulmonary health.
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Specifications

Form

Liquid

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General info

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

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Product protocols

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Target data

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
See full target information SFTPB
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