Recombinant Human Proteasome 20S beta 3 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S I M S Y N G G A V M A M K G K N C V A I A A D R R F G I Q A Q M V T T D F Q K I F P M G D R L Y I G L A G L A T D V Q T V A Q R L K F R L N L Y E L K E G R Q I K P Y T L M S M V A N L L Y E K R F G P Y Y T E P V I A G L D P K T F K P F I C S L D L I G C P M V T D D F V V S G T C A E Q M Y G M C E S L W E P N M D P D H L F E T I S Q A M L N A V D R D A V S G M G V I V H I I E K D K I T T R T L K A R M D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Alternative names
Proteasome subunit beta type-3, Proteasome chain 13, Proteasome component C10-II, Proteasome subunit beta-3, Proteasome theta chain, beta-3, PSMB3
Recommended products
Recombinant Human Proteasome 20S beta 3 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P49720-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S I M S Y N G G A V M A M K G K N C V A I A A D R R F G I Q A Q M V T T D F Q K I F P M G D R L Y I G L A G L A T D V Q T V A Q R L K F R L N L Y E L K E G R Q I K P Y T L M S M V A N L L Y E K R F G P Y Y T E P V I A G L D P K T F K P F I C S L D L I G C P M V T D D F V V S G T C A E Q M Y G M C E S L W E P N M D P D H L F E T I S Q A M L N A V D R D A V S G M G V I V H I I E K D K I T T R T L K A R M D
- Accession
- P49720
- Protein length
- Full Length
- Predicted molecular weight
- 25.1 kDa
- Amino acids
- 1 to 246
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab117227 is purified using conventional chromatography techniques.
General info
- Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
- Sequence similarities
Belongs to the peptidase T1B family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proteasome 20S beta 3 also known as PSMB3 is a structural component of the proteasome complex a multicatalytic proteinase complex with a role in degradation of ubiquitinated proteins. It has a molecular mass of approximately 25 kDa. The 20S proteasome functions in the cytoplasm and nucleus expressed in all eukaryotic cells. It participates in protein homeostasis by breaking down short-lived and damaged proteins into peptides important for cellular regulation.
- Biological function summary
Proteasome 20S beta 3 contributes to the assembly and function of the 26S proteasome complex. This complex facilitates the regulated degradation of intracellular proteins a process critical for various cellular processes including the cell cycle apoptosis and DNA repair. Proteasome 20S beta 3 is one of the 14 different subunits that make up the beta rings of the 20S core particle engaging in precise proteolytic activity within cells.
- Pathways
Proteasome 20S beta 3 is essential in the ubiquitin-proteasome pathway a major pathway for protein catabolism. This pathway maintains protein equilibrium and modulates proteins involved in immune response and signal transduction. It closely interacts with ubiquitin ligases which tag proteins for degradation and connects to key pathways like the NF-kB signaling pathway where it can influence inflammatory responses due to its role in processing specific intracellular proteins.
- Associated diseases and disorders
Proteasome 20S beta 3's dysregulation links to cancer and neurodegenerative diseases due to its role in protein degradation. In cancer altered proteasome function can result in the accumulation of oncoproteins or degradation of tumor suppressor proteins. Also disrupted protein homeostasis contributes to neurodegenerative disorders such as Alzheimer's disease where pathogenesis often involves proteasome impairment. Its interactions with proteins like the tumor suppressor p53 highlight its significance in maintaining cellular health and prevent disease progression.
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2 product images
SDS-PAGE - Recombinant Human Proteasome 20S beta 3 protein (ab117227)
15% SDS-PAGE showing ab117227 at approximately 25.1kDa (3μg).
SDS-PAGE - Recombinant Human Proteasome 20S beta 3 protein (ab117227)
SDS-PAGE analysis of ab117227 (3µg).
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