Recombinant Human Proteasome 20S C2/HC2 protein is a Human Full Length protein, in the 1 to 263 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M F R N Q Y D N D V T V W S P Q G R I H Q I E Y A M E A V K Q G S A T V G L K S K T H A V L V A L K R A Q S E L A A H Q K K I L H V D N H I G I S I A G L T A D A R L L C N F M R Q E C L D S R F V F D R P L P V S R L V S L I G S K T Q I P T Q R Y G R R P Y G V G L L I A G Y D D M G P H I F Q T C P S A N Y F D C R A M S I G A R S Q S A R T Y L E R H M S E F M E C N L N E L V K H G L R A L R E T L P A E Q D L T T K N V S I G I V G K D L E F T I Y D D D D V S P F L E G L E E R P Q R K A Q P A Q P A D E P A E K A D E P M E H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Alternative names
HC2, NU, PROS30, PSC2, PSMA1, Proteasome subunit alpha type-1, 30 kDa prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, Proteasome subunit alpha-6, PROS-30, alpha-6
Recommended products
Recombinant Human Proteasome 20S C2/HC2 protein is a Human Full Length protein, in the 1 to 263 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P25786-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M F R N Q Y D N D V T V W S P Q G R I H Q I E Y A M E A V K Q G S A T V G L K S K T H A V L V A L K R A Q S E L A A H Q K K I L H V D N H I G I S I A G L T A D A R L L C N F M R Q E C L D S R F V F D R P L P V S R L V S L I G S K T Q I P T Q R Y G R R P Y G V G L L I A G Y D D M G P H I F Q T C P S A N Y F D C R A M S I G A R S Q S A R T Y L E R H M S E F M E C N L N E L V K H G L R A L R E T L P A E Q D L T T K N V S I G I V G K D L E F T I Y D D D D V S P F L E G L E E R P Q R K A Q P A Q P A D E P A E K A D E P M E H
- Accession
- P25786
- Protein length
- Full Length
- Predicted molecular weight
- 32 kDa
- Amino acids
- 1 to 263
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab116156 is purified using conventional chromatography techniques.
General info
- Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
- Sequence similarities
Belongs to the peptidase T1A family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Proteasome 20S C2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Proteasome 20S C2/HC2 also known as the proteasome subunit beta type-1 represents an important component of the proteasome complex. This protein performs proteolytic functions degrading ubiquitinated proteins into peptides. The proteasome 20S C2/HC2 has a molecular mass of approximately 23 kDa and is expressed in various tissues throughout the body including muscle and nerve tissues. It forms part of the 20S core particle of the proteasome complex and contributes to protein homeostasis.
- Biological function summary
Proteasome 20S C2/HC2 plays a significant role in maintaining protein quality by participating in proteolytic degradation. It forms part of the larger 26S proteasome complex which assembles with regulatory particles for substrate recognition. This protein is essential for the breakdown and removal of oxidized or misfolded proteins thereby preventing the accumulation of potentially toxic aggregates within the cell. It ensures cellular regulation by processing proteins that control various aspects of cell cycle signaling and differentiation.
- Pathways
The activity of the Proteasome 20S C2/HC2 integrates directly into the ubiquitin-proteasome pathway. This pathway regulates protein turnover and plays a vital role in cellular processes such as the cell cycle and apoptosis. The association with proteins such as ubiquitin and E3 ligases is critical for the marking of substrate proteins for degradation. This protein is also linked to the NF-kB signaling pathway interacting with proteins that modulate immune and inflammatory responses demonstrating its influence on broader regulatory networks.
- Associated diseases and disorders
Proteasome 20S C2/HC2 disruption has been identified in various pathological conditions. Abnormal function or expression levels relate to neurodegenerative disorders like Parkinson’s disease where inefficient protein degradation leads to protein aggregation. This protein also connects to tumor progression in cancers where alterations in the ubiquitin-proteasome system affect cell cycle regulation and apoptosis. The protein's interaction with tumor suppressors and oncogenes highlights its significance in cellular growth abnormalities.
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1 product image
SDS-PAGE - Recombinant Human Proteasome 20S C2/HC2 protein (ab116156)
15% SDS-PAGE showing ab116156 at approximately 32 kDa (3µg).
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