JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB117226

Recombinant Human Proteasome subunit alpha type 6 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Proteasome subunit alpha type 6 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PROS27, PSMA6, Proteasome subunit alpha type-6, 27 kDa prosomal protein, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, Proteasome subunit alpha-1, PROS-27, p27K, alpha-1

1 Images
SDS-PAGE - Recombinant Human Proteasome subunit alpha type 6 protein (AB117226)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Proteasome subunit alpha type 6 protein (AB117226)

15% SDS-PAGE showing ab117226 at approximately 29.9kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P60900

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD","proteinLength":"Full Length","predictedMolecularWeight":"29.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P60900","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit alpha type 6 also known as PSMA6 is a component of the 20S core particle of the proteasome complex. This protein has a molecular mass of approximately 28.4 kDa. PSMA6 occurs in the cytoplasm and nucleus of eukaryotic cells. The proteasome functions as a protease that degrades unneeded or damaged proteins by proteolysis a chemical reaction that breaks peptide bonds. PSMA6 plays a role in the assembly and structural integrity of the proteasome.
Biological function summary

The proteasome is essential for the ubiquitin-proteasome pathway which regulates protein turnover by degrading ubiquitinated proteins. As part of the 20S core complex PSMA6 contributes to unfolding and translocating substrates into proteolytic sites. This activity helps control various cellular processes such as cell cycle progression and transcription. Abnormalities in this protein can affect normal cellular functions and regulate apoptosis and DNA repair.

Pathways

Proteasome subunit alpha type 6 is important in the Wnt signaling pathway and the NF-kB signaling pathways. In the Wnt pathway the proteasome controls the degradation of β-catenin an important effector in this signaling cascade. In the NF-kB pathway it regulates the turnover of IκB proteins repressors of NF-kB transcription factors. Together these pathways play roles in cellular proliferation differentiation and inflammation.

Abnormalities in PSMA6 have been associated with disorders such as cancer and neurodegenerative diseases. In cancer dysregulation of proteasome activity can lead to uncontrolled cell growth and resistance to apoptosis. Correlations exist between proteasome function and proteins like p53 a tumor suppressor protein. In neurodegenerative diseases like Alzheimer's proteasome impairment affects protein aggregation and degradation of misfolded proteins. Understanding PSMA6's role contributes to therapeutic developments in these conditions.
style
left-column

Specifications

Form

Liquid

Additional notes

ab117226 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Sequence similarities

Belongs to the peptidase T1A family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
See full target information PSMA6
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Primary Antibodies

AB216347

Anti-SNAIL antibody [EPR21043]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-SNAIL antibody [EPR21043] (AB216347)

  • 1
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com