Recombinant Human Protein FAM19A5 is a Human Fragment protein, in the 26 to 125 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, MS.
Images
Key facts
- Purity
- >95% Densitometry
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
Amino acid sequence
M K H H H H H H A S Q F L K E G Q L A A G T C E I V T L D R D S S Q P R R T I A R Q T A R C A C R K G Q I A G T T R A R P A C V D A R I I K T K Q W C D M L P C L E G E G C D L L I N R S G W T C T Q P G G R I K T T T V S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2 (By similarity). Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway (By similarity). Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes (By similarity). Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2 (By similarity). Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling (By similarity). Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries (By similarity).
Alternative names
FAM19A5, UNQ5208/PRO34524, TAFA5, Chemokine-like protein TAFA-5
Recommended products
Recombinant Human Protein FAM19A5 is a Human Fragment protein, in the 26 to 125 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB, MS.
Key facts
- Purity
- >95% Densitometry
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
- Accession
- Q7Z5A7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.24% Tris buffer
Sequence info
Amino acid sequence
- M K H H H H H H A S Q F L K E G Q L A A G T C E I V T L D R D S S Q P R R T I A R Q T A R C A C R K G Q I A G T T R A R P A C V D A R I I K T K Q W C D M L P C L E G E G C D L L I N R S G W T C T Q P G G R I K T T T V S
- Accession
- Q7Z5A7
- Protein length
- Fragment
- Predicted molecular weight
- 12.2 kDa
- Amino acids
- 26 to 125
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2 (By similarity). Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway (By similarity). Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes (By similarity). Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2 (By similarity). Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling (By similarity). Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries (By similarity).
- Sequence similarities
Belongs to the TAFA family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein FAM19A5 also known as TAFA5 is a member of the TAFA family and plays a role in the regulation of nervous system function. It has an approximate mass of 13 kDa and exhibits expression primarily in the central nervous system including regions such as the brain. This protein is predominantly secreted as a small cytokine-like protein where it is involved in intercellular communication within the nervous tissue.
- Biological function summary
The FAM19A5 protein influences neuroinflammatory processes and modulates the activity of neurons. While it does not form part of a large protein complex it may interact with extracellular matrix components to exert its effects. Its function is linked to the modulation of neural signaling indicating its role in maintaining homeostasis within the neural networks.
- Pathways
This protein is known to engage with critical signaling cascades implicated in the central nervous system. In particular FAM19A5 is associated with the regulation of the chemokine signaling pathway. It is linked to pathways also involving proteins such as CCR5 and CXCR3 which interact in chemotaxis and influence neural cell responses. These pathways help in communicating signals that guide neural cell behavior affecting motor coordination and cognitive functions.
- Associated diseases and disorders
Current research suggests the involvement of FAM19A5 in conditions like neurodegenerative diseases and multiple sclerosis (MS). Its function in modulating neuroinflammation connects it to these disorders which involve immune responses that damage neural tissues. FAM19A5 may interact with other immune-related proteins like cytokines contributing to the pathophysiological processes observed in these diseases. The study of FAM19A5 provides insights into therapeutic targets for the management of such neurological disorders.
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1 product image
SDS-PAGE - Recombinant Human Protein FAM19A5 (ab203547)
14% SDS-PAGE separation of ab203547.
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and heated sample, 2.5μg / lane
3. Non-reduced and non-heated sample, 2.5μg / laneab203547 migrates as two significant bands corresponding to molecular mass 10kDa and 14kDa, respectively. Both bands were confirmed by LC-MS/MS and identified as FAM19A5.
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